TRYPSIN CLEAR™

Mass Spectrometry Grade

Chemically modified and highly pure protease for bottom-up proteomics with high specificity and enhanced stability.

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“HIGHLY SPECIFIC DIGESTION FOR CLEAR RESULTS IN YOUR PROTEOMICS ANALYSIS”

TRYPSIN CLEAR ™

Digestion of samples with Trypsin Clear™ yields cleaner mass spectra and clearer results: clear from spectrum-noise, clear from non-specific activity, and clear from autodigestion.

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Product overview

Description

Trypsin Clear™ is a serine protease that provides highly specific digestion for clearer proteomics analysis. It has been carefully developed to yield mass spectrometry results without background noise or non-desired peptide fragments. 

Trypsin-Clear™ hydrolyze protein by cleaving the peptide bonds at the C-terminal of lysine and arginine residues with superior performance. It has been chemically modified and treated with TPCK to make it exceptionally resistant to autolytic digestion and suppress any remaining chymotryptic activity. These treatments together with a high purification process boost trypsin stability, specificity and purity, which translates into mass spectra without the interference of autolytic and chymotryptic peptides.

Clear Results: clear from noise and non-desire peptides for better proteomic analysis. 

Highly Pure: multiple chromatographic purification steps to eliminate any contamination.

Superior Specificity: an extremely sequence-specific enzyme with minimized chymotryptic activity

Enhanced Stability: chemically modified to be resistant to autodigestion.

Specifications

EC Number: 3.4.21.4

CAS number: 9002-07-7

Product Formats:  100 μg  or  5x20 μg

Product Form: Lyophilized

Optimal pH Range: 7.0 - 9.0

Digestion Temperature: 37°C

Storage: Store at -20°C.

The products are shipped at 2-8°C. Upon reception store at -20°C.

Stability: The lyophilized powder is stable for 2 years at -20°C. Use only freshly reconstituted trypsin for best results.

Activity: > 10,000 U/vial

Unit Definition: One unit (U) of enzyme activity is defined as the amount of enzyme capable of hydrolyzing 1 μmol of BApNA in 30 min at 25°C and pH 8.0.

Available Presentations

    100 μg      5x20 μg 

Resources

Technical Datasheets

Technical Datasheet Trypsin Clear

Others

Trypsin Clear Brochure

 

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