Kura Biotech's enzyme generates more homogeneous products, requires less template to amplify, and also needs shorter extension times in each amplification cycle. Therefore, it is more efficient and has proofreading activity that enables the generation of more homogeneous products.

I just tested DeCodi-Fi and compared it to the one I regularly use in the lab for cloning. The yield (amount of product) is higher, and without a doubt, I can say that I like the product because it is so simple and fast to work with, while also giving me confidence that it is a high-fidelity enzyme. The kit is very easy to use since everything comes ready to go, which is really convenient.

I want to highlight the speed of the PCR—it shortens the reaction time by about 20–30 minutes. The PCR product yield is great, making it easy to identify the desired fragment. I'm very satisfied with the product. One of the diseases I study involves a candidate gene with high GC content and pseudogenes, which makes PCR quite challenging. I work with limited amounts of DNA and still achieve high yields of the desired product. When dealing with patient samples, where material is low, that's a major advantage.

Kura Biotech's enzyme is of excellent quality and surpasses many of the options currently available on the market. The objective of the experiment was to generate a plasmid encoding a truncated protein using the overlap PCR technique. The results with DeCodi-Fi were very positive: we managed to correctly generate the desired plasmid, and the PCRs were fast and consistent. No errors or aberrant products were observed at any point in the process. This experiment was performed in parallel with a Pfu enzyme. While both enzymes worked correctly, DeCodi-Fi showed a clear advantage: it did not generate non-specific fragments, unlike the Pfu, which did produce unwanted products of different sizes.