Thermostability: Functions efficiently at different temperatures.
Accel TdT Program
CUSTOMIZED TDT ENZYMES
FOR DNA AND RNA SYNTHEIS
Start with our proprietary TdT Synthesis Panel and leverage Cantera, our protein engineering platform, for tailored solutions.
Accel TdT Program
CUSTOMIZED TDT ENZYMES FOR DNA AND RNA SYNTHEIS
Start with our proprietary TdT Synthesis Panel and leverage Cantera, our protein engineering platform, for tailored solutions.
What we offer
At Blikka Genomics, we are transforming the landscape of DNA and RNA synthesis. Our journey begins with the TdT Synthesis Panel, leading into the Accel-TdT Program where we customize TdT enzymes tailored to your specific DNA synthesis protocols using our advanced protein engineering platform, Cantera.
What we offer
At Blikka Genomics, we are transforming the landscape of DNA and RNA synthesis. Our journey begins with the TdT Synthesis Panel, leading into the Accel-TdT Program where we customize TdT enzymes tailored to your specific DNA synthesis protocols using our advanced protein engineering platform, Cantera.
- Proprietary Portfolio of TdT Enzymes Ready for Evolution:
Commercially available TdT enzymes are not optimized for non-native nucleotide analogs needed for controlled synthesis of DNA/RNA sequences. Blikka Genomics offers a diverse portfolio of proprietary Terminal Deoxynucleotidyl Transferase (TdT) enzymes sourced from artificial chimeric origins. These sequences serve as a robust backbone that can be evolved and tailored to meet specific needs. - Protein Engineering Platform:
Our specialized protein engineering platform, Cantera, leverages over 11 years of experience, employing a structured, data-driven process to generate and optimize enzyme candidates. By integrating deep sequencing for faster learning cycles, Cantera ensures efficient production and delivery of final enzyme products. - OEM Licensing and Custom Manufacturing:
Full access to our proprietary library of TdTs with OEM licensing agreements. Have enzymes manufactured at Blikka with custom labeling requirements or opt for manufacturing at your own facility, integrating advanced enzymatic solutions seamlessly into your production processes. - Sustainability Impact:
The Accel-TdT Program supports sustainable biotechnology by reducing harmful chemical waste and lowering energy consumption, compared to traditional chemical synthesis methods. This not only enhances your research but also contributes to a more sustainable future.
Technical Data Sheet
TdT Synthesis Panel TDS
Brochure
TdT Synthesis Panel Brochure
- Proprietary Portfolio of TdT Enzymes Ready for Evolution:
Commercially available TdT enzymes are not optimized for non-native nucleotide analogs needed for controlled synthesis of DNA/RNA sequences. Blikka Genomics offers a diverse portfolio of proprietary Terminal Deoxynucleotidyl Transferase (TdT) enzymes sourced from artificial chimeric origins. These sequences serve as a robust backbone that can be evolved and tailored to meet specific needs. - Protein Engineering Platform:
Our specialized protein engineering platform, Cantera, leverages over 11 years of experience, employing a structured, data-driven process to generate and optimize enzyme candidates. By integrating deep sequencing for faster learning cycles, Cantera ensures efficient production and delivery of final enzyme products. - OEM Licensing and Custom Manufacturing:
Full access to our proprietary library of TdTs with OEM licensing agreements. Have enzymes manufactured at Blikka with custom labeling requirements or opt for manufacturing at your own facility, integrating advanced enzymatic solutions seamlessly into your production processes. - Sustainability Impact:
The Accel-TdT Program supports sustainable biotechnology by reducing harmful chemical waste and lowering energy consumption, compared to traditional chemical synthesis methods. This not only enhances your research but also contributes to a more sustainable future.
Technical Data Sheet
TdT Synthesis Panel TDS
Brochure
TdT Synthesis Panel Brochure
HOW THE
ACCEL TDT
PROGRAM
WORKS
Step 1
Test a TdT
Begin by testing a TdT from our TdT Synthesis Panel. You can order the TdT as it is in custom-size formats, or evolve it.
Step 2
Data Gathering
We start by collecting comprehensive data from scientific literature, databases, experiments, and advanced bioinformatics tools to identify key residues for modification.
Step 3
Library Construction
We generate diverse variants in silico to be tested in the lab.
Step 4
High Throughput Screening
We produce and screen mutants in the lab to identify the best variants.
Step 5
Selection of Best Mutants
We conclude by conducting an in-depth characterization of top enzymes to evaluate performance metrics such as activity, stability, and kinetics.
HOW THE
ACCEL TDT
PROGRAM
WORKS
Step 1
Test a TdT
Begin by testing a TdT from our TdT Synthesis Panel. You can order the TdT as it is in custom-size formats, or evolve it.
Step 2
Gathering Data
We start by collecting comprehensive data from scientific literature, databases, experiments, and advanced bioinformatics tools to identify key residues for modification.
Step 3
Library Construction
We generate diverse variants in silico to be tested in the lab.
Step 4
High Throughput Screening
We produce and screen mutants in the lab to identify the best variants.
Step 5
Selection of Best Mutants
We conclude by conducting an in-depth characterization of top enzymes to evaluate performance metrics such as activity, stability, and kinetics.
THE STARTING POINT
TDT SYNTHESIS PANEL
Our versatile panel offers 6 proprietary and engineered Terminal Deoxynucleotidyl Transferase (TdT) enzymes, designed for high coupling efficiency with both natural and modified nucleotides, range of thermostability, and low bias.
These enzymes serve as a foundation to be tested, and the one that works best for customization in co-development with our Product Engineering team is selected.
Key Features
Compatibility with Blocked Nucleotides: Works effectively with 3’O-NH2 reversibly blocked dNTPs.
High Coupling Efficiency & Low Bias: Delivers excellent coupling efficiency and low bias with natural DNA nucleotides, modified DNA nucleotides, and natural RNA nucleotides, ensuring high-quality synthesis outcomes.
pH Resistance: Stable and functional across a broad pH range (pH 5-9), providing flexibility in various synthesis conditions.
THE STARTING POINT
TDT SYNTHESIS PANEL
Our versatile panel offers 6 proprietary and engineered Terminal Deoxynucleotidyl Transferase (TdT) enzymes, designed for high coupling efficiency with both natural and modified nucleotides, range of thermostability, and low bias.
These enzymes serve as a foundation to be tested, and the one that works best for customization in co-development with our Product Engineering team is selected.
Key Features
Thermostability: Functions efficiently at different temperatures.
Compatibility with Blocked Nucleotides: Works effectively with 3’O-NH2 reversibly blocked dNTPs.
High Coupling Efficiency & Low Bias: Delivers excellent coupling efficiency and low bias with natural DNA nucleotides, modified DNA nucleotides, and natural RNA nucleotides, ensuring high-quality synthesis outcomes.
pH Resistance: Stable and functional across a broad pH range (pH 5-9), providing flexibility in various synthesis conditions.
PERFORMANCE DATA
DIVERSITY IN TdT BACKGROUNDS +
6 TdT from different sources Tailored to precisely match your unique requirements, ensuring the best possible fit for you.
INCORPORATION OF DNA NATURAL NUCLEOTIDES +
Addition of natural deoxyribonucleotides. Enzymes were incubated with a 1:10 oligo:dNTP ratio for 1 minute at 37°C.
INCORPORATION OF DNA-MODIFIED NUCLEOTIDES +
Addition of modified nucleotides. Enzymes were incubated with a 1:10 oligo:dNTP ratio for 30 minutes at 37°C. Bovine TdT didn’t show activity.
INCORPORATION OF RNA NATURAL NUCLEOTIDES +
Addition of natural ribonucleotides. Enzymes were incubated with a 1:10 oligo:dNTP ratio for 30 minutes at 37°C.
Ready to Transform Your Synthesis Process?
Take the first step towards customizing TdT enzymes for your DNA and RNA synthesis needs. Leverage our proprietary TdT Synthesis Panel and partner with our expert team through the Accel TdT Program to create tailored solutions that enhance your research.
Ready to Transform Your Synthesis Process?
Take the first step towards customizing TdT enzymes for your DNA and RNA synthesis needs. Leverage our proprietary TdT Synthesis Panel and partner with our expert team through the Accel TdT Program to create tailored solutions that enhance your research.