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APPLICATIONS:

DNA Library Amplification and High Fidelity PCR

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Polymerase Chain Reaction (PCR) is a technique used to amplify DNA, generating millions of copies from a small initial sample. This method is essential for detecting or studying specific genes, DNA regions, or mutations when the available DNA is insufficient for analysis. PCR multiplies the DNA, producing sufficient copies for analysis. It is used in applications such as cloning, diagnostic testing, and sequencing.

Successful sequencing relies on the quality of library preparation workflows. The choice of polymerase is crucial, high-fidelity enzymes ensure minimal errors during DNA amplification, enabling accurate sequencing results. For Next Generation Sequencing (NGS) applications, high-fidelity polymerases with proofreading activity correct mismatches, maintaining the integrity of the amplified DNA and ensuring reliable downstream analysis.

 

DeCodi-Fi, the high-fidelity polymerase from Blikka Genomics, offers high performance at a more affordable price than other options on the market. It provides higher fidelity and yield, longer fragment amplification, and greater coverage, making it an excellent choice for NGS library preparation. With enhanced accuracy and robust performance, DeCodi-Fi enables researchers to optimize their workflows, achieve precise results, and expand their sequencing capabilities.

 

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DeCodi-Fi's Performance Data

High-Fidelity

DeCodi-Fi High-Fidelity Polymerase exhibits a 64% reduction in error rates compared to KAPA HiFi.

 

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Comparison of DeCodi-Fi, KAPA HiFi, and Toyobo HiFi, measured in terms of fold improvement over Taq Polymerase, based on Illumina libraries of the complete E. coli genome.

Long Amplification & High Yield

 

Maximize target yield with highly efficient amplification, even with fragment sizes up to 44 kb of length.

 

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Amplification of lambda DNA fragments 20 kb and 44 kb with DeCodi-Fi  High-Fidelity polymerase, KAPA HiFi, Platinum SuperFi, and Q5. Each target was amplified from low input (1ng)  Reactions were performed following the optimized conditions for each enzyme and using 24 cycles. The experiment was run in triplicate.

High Yield

 

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DNA concentration obtained from 25 μL of lambda 15 kb PCR reaction using DeCodi-Fi PCR kit, DeCodi-Fi All-in-One Mix, and competitors enzymes (Kapa HiFi, Platinum SuperFi, and Q5). The experiment was run in triplicate.

DeCodi-Fi High-Fidelity Polymerase Applications

Amplification of long or GC-rich templates

Library amplification for sequencing

High Fidelity and Specificity PCR

High Throughput PCR

DNA methylation research 

Uracil excision protocols (eg. USER cloning)

Resources

Technical Data Sheet
down DeCodi-Fi All-In-One Mix TDS
down DeCodi-Fi PCR Kit TDS

Brochure
down DeCodi-Fi High-Fidelity Polymerase Brochure
 
Poster
down Unveiling Microbial Diversity Cost-Effective 16S rRNA Amplicon Sequencing using DeCodi-Fi High Fidelity Polymerase
down DeCodi-Fi: A Cost-Effective Polymerase for High-Quality Genome Assembly of Marine Sediment Bacteria
Application Note
down Resistance of High-Fidelity DNA polymerases to magnetic beads' presence

Ready to Amplify Your Research?

Take your DNA sequencing to the next level with DeCodi-Fi.
Contact our team for sales and technical support.

Ready to Amplify Your Research?

Take your DNA sequencing to the next level with DeCodi-Fi.
Contact our team for sales and technical support.