DeCodiFi™

Choose DeCodiFi™ when your workflow requires high-fidelity and/or long amplification with strong yield, low-bias coverage, fast cycling, low-input compatibility, and flexible format options for PCR or sequencing library preparation workflows.

Choose DeCodiFi™ 2X All-In-One Mix for simple setup, fewer pipetting steps, routine workflows, and higher-throughput use.

Choose DeCodiFi™ High-Fidelity PCR Kit when you need more optimization flexibility. Use the High-Fidelity Buffer when maximum fidelity is the priority or when working with balanced GC/AT templates. Use the GC-rich Buffer when amplifying high-GC amplicons, up to 80% GC.

A Non-Hot-Start version is also available on request for custom workflow needs.

For DNA fragments under 1 kb, DeCodiFi™ typically requires 30 seconds of extension. For larger fragments, add 15 seconds per additional kilobase. For example, a 23 kb fragment would require approximately 6 minutes of extension.

DeCodiFi™ supports amplification from low input amounts, including high-complexity DNA from 0.1 ng and medium-complexity templates from 5 pg. As a general starting point, use 5–50 ng for genomic DNA and less than 1 ng for templates smaller than 50 kb. Exact input should be optimized based on template complexity, target size, and application.

We recommend starting with 25 cycles for most PCR workflows when input is above 10⁴ template copies. Keep cycling under 30 cycles, especially for amplicons larger than 10 kb. For Illumina sequencing libraries below 600 bp, 12–14 cycles can be used as a starting point when beginning from 1 ng of template.

DeCodiFi™ HotStart High-Fidelity Polymerase is inactivated by an antibody until the initial thermal denaturation step. This helps prevent non-specific priming during reaction setup and improves amplification specificity.

Yes. DeCodiFi™ is designed to read through and amplify uracil-containing DNA, supporting workflows such as methylation analysis, damaged DNA amplification, and protocols involving uracil-containing primers or templates.

Because DeCodiFi™ has strong proofreading activity, we recommend adding two phosphorothioate bonds at the 3′ end of primers to help prevent 3′ exonuclease degradation. This is especially important for shorter primers and NGS library amplification workflows. Refer to the protocol for examples and ordering notation.

Yes. A Non-Hot-Start version of DeCodiFi™ is available on request and can be customized to specific workflow needs.

Yes. Custom and OEM solutions are available for teams that need enzyme optimization, buffer refinement, custom formulations, or manufacturing support.

Store DeCodiFi™ at -20°C immediately upon receipt and avoid repeated freeze-thaw cycles. DeCodiFi™ is designed to be transported at 2°C to 8°C without performance loss, with stability confirmed under those conditions for at least 10 days during transport.