DeCodiFi™ generates more homogeneous products, requires less template to amplify, and also needs shorter extension times in each amplification cycle. Therefore, it is more efficient and has proofreading activity that enables the generation of more homogeneous products.

I just tested DeCodiFi™ and compared it to the one I regularly use in the lab for cloning (Phusion High-Fidelity DNA Polymerase from Thermo). The yield (amount of product) is higher than with Phusion, and without a doubt, I can say that I like the product because it is so simple and fast to work with, while also giving me confidence that it is a high-fidelity enzyme. Your kit is very easy to use since everything comes ready to go, which is really convenient.

I want to highlight the speed of the PCR—it shortens the reaction time by about 20–30 minutes. The PCR product yield is great, making it easy to identify the desired fragment. I'm very satisfied with the product. One of the diseases I study involves a candidate gene with high GC content and pseudogenes, which makes PCR quite challenging. I work with limited amounts of DNA and still achieve high yields of the desired product. When dealing with patient samples, where material is low, that's a major advantage.

DeCodiFi™ is of excellent quality and surpasses many of the options currently available on the market. The objective of the experiment was to generate a plasmid encoding a truncated protein using the overlap PCR technique. The results with DeCodiFi™ were very positive: we managed to correctly generate the desired plasmid, and the PCRs were fast and consistent. No errors or aberrant products were observed at any point in the process. This experiment was performed in parallel with a Pfu enzyme. While both enzymes worked correctly, DeCodiFi™ showed a clear advantage: it did not generate non-specific fragments, unlike the Pfu, which did produce unwanted products of different sizes.

I have now evaluated the DeCodiFi™ polymerase extensively. For long PCR products, it demonstrates both improved performance and faster amplification compared to our current Promega and Emerald long-range polymerases. Its advantages are particularly evident in cDNA long-read sequencing applications. In our workflow, we amplify full-length mRNA transcripts, with a focus on low-abundance, long, and complex genes. Using DeCodiFi™, we were able to reduce our wet-lab protocol from two days to a single day due to its high amplification speed, which is especially valuable in time-sensitive settings. In addition, DeCodiFi™ nearly doubled our sequencing depth compared to NEB LongAmp. More importantly, it enabled the consistent recovery of full-length transcript reads, which had previously been challenging to obtain. Overall, the use of DeCodiFi™ has significantly improved the quality and efficiency of our cDNA long-read sequencing experiments.