DeCodiFi™ Blood Direct is a hot-start high-fidelity 2X All-In-One Mix designed for direct PCR from whole blood, supporting amplification from fresh blood, preserved samples, and dried blood spots while reducing DNA extraction steps.
Direct PCR from whole blood
Tolerates blood-derived inhibitors
Compatible with fresh, preserved, and dried blood samples
Supports long-fragment PCR from blood
Skip DNA purification
Formats: 2X All-In-One Mix
Supports high-fidelity PCR directly from whole blood, helping reduce DNA extraction steps.
Designed to tolerate common blood-derived PCR inhibitors, including EDTA, citrate, and heparin.
Compatible with fresh blood, preserved blood samples, and dried blood spots stored on filter paper.
Supports robust amplification of long genomic targets from blood samples containing inhibitors.
Designed to support amplification from limited or degraded DNA quantities.
Helps reduce sample preparation time by enabling direct amplification from blood-derived samples.
PERFORMANCE DATA
DeCodiFi™ Blood Direct supports direct PCR amplification from human whole blood samples preserved with EDTA, citrate, or heparin.
Comparison of direct PCR amplification (template size: 584bp) across various samples of human blood preserved with EDTA, citrate, and heparin using DeCodi-Fi™ Blood Direct and Q5 Blood Direct.
DeCodiFi™ Blood Direct supports long-fragment amplification from whole blood under tested conditions.
Comparison of direct PCR amplification of a human genomic amplicon of 17kb from 10% EDTA-preserved human whole blood using DeCodi-Fi™ Blood Direct and Q5 Blood Direct.
APPLICATIONS
For workflows that require PCR directly from whole blood while reducing DNA extraction steps.
For blood genotyping PCR workflows where sample preparation speed and consistent amplification are important.
For blood-derived samples where EDTA, citrate, heparin, or other sample components can interfere with PCR.
For blood amplification workflows where amplification accuracy is important.
