
From field to PCR. Skip the purification.
DeCodi-Fi™ Blood Direct is a 2X All-in-One Mix designed to enable high-fidelity PCR directly from whole blood, whether fresh, preserved, or dried on filter paper. Its proprietary buffer formulation tolerates common PCR inhibitors like EDTA, citrate, and heparin, eliminating the need for DNA extraction. Powered by the DeCodi-Fi™ high-fidelity polymerase, it delivers the accuracy, yield, and consistency that define the DeCodi-Fi™ kit family. Ideal for genotyping, forensic analysis, and clinical workflows requiring fast, extraction-free PCR.

From field to PCR. Skip the purification.
DeCodi-Fi™ Blood Direct is a 2X All-in-One Mix designed to enable high-fidelity PCR directly from whole blood, whether fresh, preserved, or dried on filter paper. Its proprietary buffer formulation tolerates common PCR inhibitors like EDTA, citrate, and heparin, eliminating the need for DNA extraction. Powered by the DeCodi-Fi™ high-fidelity polymerase, it delivers the accuracy, yield, and consistency that define the DeCodi-Fi™ kit family. Ideal for genotyping, forensic analysis, and clinical workflows requiring fast, extraction-free PCR.
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High inhibitor tolerance: Handles blood-derived inhibitors like EDTA, citrate, and heparin, enabling direct PCR with no purification required.
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Sample flexibility: Validated for fresh blood, preserved samples, and dried blood spots stored on common filter papers.
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Low input: Delivers consistent results even from limited or degraded DNA quantities.
- Genotyping by sequencing
- Multiplex PCR
-
High inhibitor tolerance: Handles blood-derived inhibitors like EDTA, citrate, and heparin, enabling direct PCR with no purification required.
-
Sample flexibility: Validated for fresh blood, preserved samples, and dried blood spots stored on common filter papers.
-
Low input: Delivers consistent results even from limited or degraded DNA quantities.
- Genotyping by sequencing
- Multiplex PCR
KEY PERFORMANCE DATA
AMPLIFY DIRECTLY FROM HUMAN WHOLE BLOOD +
Consistent PCR performance from samples preserved with EDTA, citrate, and heparin.
Comparison of direct PCR amplification (template size: 584bp) across various samples of human blood preserved with EDTA, citrate, and heparin using DeCodi-Fi™ Blood Direct and Q5 Blood Direct.
ROBUST PERFORMANCE WITH LONG FRAGMENTS +
Consistently amplifies long genomic targets from blood with inhibitors, outperforming competitor polymerases.
Comparison of direct PCR amplification of a human genomic amplicon of 17kb from 10% EDTA-preserved human whole blood using DeCodi-Fi™ Blood Direct and Q5 Blood Direct.
PRODUCT FORMAT

DeCodi-Fi™ Blood Direct
2X All-In-One Mix![]()
Provides our DeCodi-Fi™ high-fidelity polymerase in a 2X All-in-One MasterMix optimized for whole blood samples. This ready-to-use mix contains all essential PCR components: dNTPs, MgCl₂, and stabilizers, within a proprietary buffer formulated to tolerate common blood inhibitors such as EDTA, citrate, and heparin. Just add primers and sample.
Product Type: All-in-One/MasterMix
Polymerase: DeCodi-Fi™ Hotstart High-Fidelity Polymerase
Format: 5 mL
Reactions: 400
Storage: Store at -20°C
RELATED PRODUCTS
Ready to amplify your research?
Take your DNA sequencing to the next level with DeCodi-Fi™. Contact our team for sales and technical support.
Ready to amplify your research?
Take your DNA sequencing to the next level with DeCodi-Fi.
Contact our team for sales and technical support.




