This TdT was designed to incorporate 3′-amino blocked nucleotides, which makes it well-suited for workflows that rely on reversible blocking strategies.
Relevant for methods where nucleotide addition needs to be compatible with iterative or stepwise workflows, such as controlled tailing and enzymatic DNA synthesis methods.
Work directly with our team to discuss nucleotide chemistry, reaction conditions, and next-step optimization.
PERFORMANCE DATA
DeCodiFi™ Alpha shows an ultra-high fidelity profile in an Illumina library-based comparison of polymerase error rates, supporting workflows where polymerase-introduced errors can affect downstream interpretation.
HIGHER FIDELITY
Comparison of DeCodiFi™ Alpha 2X All-In-One Mix, Kura DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, Watchmaker Equinox Library Amplification Kit, Roche KAPA HiFi HotStart ReadyMix, and Taq, measured in error per million based on Illumina libraries of the complete E. coli genome.
DeCodiFi™ Alpha supports uniform coverage across tested genomic regions, helping reduce GC-related coverage bias during NGS library amplification.
UNIFORM COVERAGE
Illumina libraries prepared from an E. coli genome were amplified using DeCodiFi™ Alpha 2X All-In-One Mix, DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, and Taq polymerase.
APPLICATIONS
For synthesis workflows that require repeatable TdT performance across multiple extension cycles.
For workflows that depend on efficient one-by-one incorporation of 3′-NH₂ blocked dNTPs and reversible blocking strategies.
