
Amplify what others can't.
DeCodi-Fi™ Long&Complex is an All-in-One Mix optimized for the toughest PCR targets: long fragments, ultra-GC-rich regions, and repetitive sequences. Powered by the DeCodi-Fi™ high-fidelity polymerase, it delivers the precision, yield, and consistency that define the DeCodi-Fi™ kit family. Built for long-read sequencing, gene synthesis, and complex genomic assemblies, it enables reliable and accurate amplification even from low-input complex samples where other polymerases fail.

Amplify what others can't.
DeCodi-Fi™ Long&Complex is an All-in-One Mix optimized for the toughest PCR targets: long fragments, ultra-GC-rich regions, and repetitive sequences. Powered by the DeCodi-Fi™ high-fidelity polymerase, it delivers the precision, yield, and consistency that define the DeCodi-Fi™ kit family. Built for long-read sequencing, gene synthesis, and complex genomic assemblies, it enables reliable and accurate amplification even from low-input complex samples where other polymerases fail.
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Long complex fragments: Robust amplification of fragments up to 30 kb from human genomic DNA.
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High GC content tolerance: Efficient amplification of targets with up to 90% GC content.
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Performance on repeat regions: Reliable performance on challenging GC-rich repeat regions.
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Low input: High sensitivity, works with as little as 100 pg of input DNA.
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Fast cycling: Fast extension speeds, less than 15 seconds per kb.
- High performance in long-read sequencing library amplification: Achieving higher mean fragment length and higher contig N50.
- Library amplification for long read sequencing
- Amplification of GC-rich templates and repetitive regions
- Amplification of complex genomic regions for variant analysis
- Amplification of long or structured DNA for cloning and assembly
- Amplification of low-input for sensitive workflows
-
Long complex fragments: Robust amplification of fragments up to 30 kb from human genomic DNA.
-
High GC content tolerance: Efficient amplification of targets with up to 90% GC content.
-
Performance on repeat regions: Reliable performance on challenging GC-rich repeat regions.
-
Low input: High sensitivity, works with as little as 100 pg of input DNA.
-
Fast cycling: Fast extension speeds, less than 15 seconds per kb.
- Library amplification for long read sequencing
- Amplification of GC-rich templates and repetitive regions
- Amplification of complex genomic regions for variant analysis
- Amplification of long or structured DNA for cloning and assembly
- Amplification of low-input for sensitive workflows
KEY PERFORMANCE DATA
EXTREME GC CAPABILITY +
Reliably amplifies templates up to 90% GC, achieving higher yield and half the extension time.
Amplification of the 10 kb human IGF2R gene, containing a 90% GC-rich region, using DeCodi-Fi™ Long&Complex and KOD Xtreme™ polymerases. Reactions were performed from 10ng of input DNA under each enzyme’s optimized conditions, with annealing temperatures of 58°C, 60°C, and 62°C, and 28 PCR cycles.
LONG AMPLIFICATION +
Robust amplification of 30 kb human fragments, delivering cleaner profiles with minimal background.
Amplification of human DNA 30 kb with DeCodi-Fi™ Long&Complex, KOD Xtreme™, KOD One™, and PrimeSTAR GXL. Each target was amplified from 10 ng of input. Reactions were performed following the optimized conditions for each enzyme and using 30 cycles.
HIGH-COMPLEXITY ACCURACY +
Clean, defined bands for 20 kb with 70% GC fragments, outperforming competitors in clarity and specificity.
Amplification of Streptomyces leeuwenhoekii gDNA 20kb with DeCodi-Fi™ Long&Complex, KODXtremeTM, KOD One™, and PrimeSTAR GXL. All PCRs contained 4ng of input. Reactions were performed following the optimized conditions for each enzyme and using 27 cycles.
LONG-READ SEQUENCING LIBRARY AMPLIFICATION +
Robust long-read library amplification delivering strong sequencing metrics across genomes with varying GC content: Escherichia coli (~50–51% GC) and Staphylococcus epidermidis (~32–33% GC).
Table 1: Sequencing and assembly metrics for E.coli library
| Escherichia coli | >Q30 (%) | Mean length | N50 | Gene completeness |
| KOD Xtreme | 97 | 5.9 kb | 3,539 kb | 92.47% |
| DeCodi-Fi™ Long&Complex | 97 | 6.7 kb | 4,585 kb | 92.40% |
| Q5 | 98 | 4.3 kb | 1,610 kb | 92.37% |
| PrimeSTAR GXL | 97 | 5.4 kb | 4,585 kb | 92.43% |
Table 2: Sequencing and assembly metrics for S.epidermis library
| Staphylococcus epidermis | >Q30 (%) | Mean length | N50 | Gene completeness |
| KOD Xtreme | 97 | 7.9 kb | 1,673 kb | 98.40% |
| DeCodi-Fi™ Long&Complex | 97 | 8.5 kb | 2,145 kb | 98.37% |
| Q5 | 98 | 5.6 kb | 1,716 kb | 98.43% |
| PrimeSTAR GXL | 98 | 6.4 kb | 2,142 kb | 98.40% |
Library amplification of E. coli and S. epidermidis genomes shows that polymerase processivity directly impacts assembly performance. DeCodi-Fi™ Long&Complex achieved the highest mean read lengths (6.7 kb and 8.5 kb) and the highest N50 values (4,585 kb and 2,145 kb), with gene completeness of ~98%. Overall, processivity emerges as the key driver for optimal long-read assembly in ultra-low input workflows.
PRODUCT FORMAT

DeCodi-Fi™ Long&Complex
2X All-In-One Mix![]()
Provides our DeCodi-Fi™ high-fidelity polymerase in a 2X MasterMix optimized for complex templates. This mix includes all essential PCR components: dNTPs, MgCl₂, and stabilizers, within a proprietary buffer designed to enhance performance on long and GC-rich targets. Just add primers and template.
Product Type: All-in-One/MasterMix
Polymerase: DeCodi-Fi™ Hotstart High-Fidelity Polymerase
Format: 5 mL
Reactions: 400
Storage: Store at -20°C
RELATED PRODUCTS
Ready to amplify your research?
Take your DNA sequencing to the next level with DeCodi-Fi™. Contact our team for sales and technical support.
Ready to amplify your research?
Take your DNA sequencing to the next level with DeCodi-Fi.
Contact our team for sales and technical support.



