DeCodiFi™ Alpha

Name: DeCodiFi Alpha High-Fidelity Polymerase
Brand: Kura Biotech
SKU: 22800

Accurate Library Prep & High-Fidelity Amplification

Ultra-High Fidelity NGS Master Mix

2X All-In-One Master Mix built for short-read NGS library amplification, combining ultra-high fidelity, uniform coverage, low-input sensitivity, and overcycling stability to help reduce polymerase-introduced error and amplification bias.

Ultra-high fidelity profile

Uniform coverage

Overcycling stability

Fast cycling: under 15 sec/kb

Robust library yields from 5 pg of DNA

High DNA recovery

Format: 2X All-In-One Mix

Request a quote

Most “high-fidelity” polymerases force a compromise: accuracy or coverage. But in short-read NGS library amplification, that tradeoff can shape what you see downstream.

DeCodiFi™ Alpha is built for researchers who know that a single PCR error, a coverage dropout, or amplification bias in a challenging genomic region can change the story of a library. Engineered for demanding short-read applications, Alpha combines ultra-high fidelity with uniform coverage, high DNA recovery, and low-input sensitivity to help preserve library representation across complex genomic landscapes.

By supporting coverage uniformity across AT- and GC-rich regions, DeCodiFi™ Alpha helps reduce polymerase-introduced error, amplification artifacts, and library representation bias, turning precious low-input samples into high-quality sequencing-ready data.

Ultra-high fidelity

Built to reduce polymerase-introduced errors in NGS library amplification workflows.

Uniform coverage

Designed to maintain even depth across genomic landscapes and reduce amplification bias.

Overcycling stability

Helps preserve library complexity and reduce sequence dropout risk when additional cycles are needed.

Low-input sensitivity

Supports robust library yields from as little as 5 pg DNA under tested conditions.

High yield

Delivers high DNA recovery for sequencing-ready libraries.

Fast cycling

Supports fast extension rates under 15 sec/kb.

Product format

OPTIMIZED FOR ACCURATE LIBRARY AMPLIFICATION

DeCodi-Fi™ Alpha
2X All-In-One Mix

Provides our DeCodi-Fi™ high-fidelity polymerase in a 2X MasterMix optimized for NGS library amplification. This mix includes all essential PCR components: dNTPs, MgCl₂, and stabilizers, within a proprietary buffer designed to enhance fidelity and coverage. Just add primers and template.

Product Type: All-In-One / MasterMix
Polymerase: DeCodiFi™ Alpha Hotstart High-Fidelity Polymerase
Format: 5 mL
Reactions: 400
Storage: -20°C

Request a quote

Product format

OPTIMIZED FOR ACCURATE LIBRARY AMPLIFICATION

DeCodiFi™ Alpha

2X All-In-One Mix
Provides our DeCodiFi™ high-fidelity polymerase in a 2X MasterMix optimized for NGS library amplification. This mix includes all essential PCR components: dNTPs, MgCl₂, and stabilizers, within a proprietary buffer designed to enhance fidelity and coverage. Just add primers and template.

Request a quote

Product Type: All-in-One/MasterMix

Polymerase: DeCodiFi™ Alpha Hot-Start High-Fidelity Polymerase

Format: 5 mL

Reactions: 400

Storage: -20°C

PERFORMANCE DATA

Evidence-based performance

Polymerase error rate in Illumina library amplification

DeCodiFi™ Alpha shows an ultra-high fidelity profile in an Illumina library-based comparison of polymerase error rates, supporting workflows where polymerase-introduced errors can affect downstream interpretation.

HIGHER FIDELITY

Comparison of Kura DeCodiFi™ Alpha 2X All-In-One Mix, Kura DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, Watchmaker Equinox Library Amplification Kit, Roche KAPA HiFi HotStart ReadyMix, and Taq, measured in error per million based on Illumina libraries of the complete E. coli genome.

Comparison of DeCodiFi™ Alpha 2X All-In-One Mix, Kura DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, Watchmaker Equinox Library Amplification Kit, Roche KAPA HiFi HotStart ReadyMix, and Taq, measured in error per million based on Illumina libraries of the complete E. coli genome.

Coverage uniformity across GC content

DeCodiFi™ Alpha supports uniform coverage across tested genomic regions, helping reduce GC-related coverage bias during NGS library amplification.

UNIFORM COVERAGE
Illumina libraries prepared from an E. coli genome were amplified using Kura DeCodiFi™ Alpha 2X All-In-One Mix, Kura DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, and Taq polymerase.

Illumina libraries prepared from an E. coli genome were amplified using DeCodiFi™ Alpha 2X All-In-One Mix, DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, and Taq polymerase.

APPLICATIONS

Sequencing & Library Preparation

DeCodiFi™ Alpha supports sequencing library preparation workflows where ultra-high fidelity, uniform coverage, low-input sensitivity, and library complexity are important for sequencing-ready DNA.

NGS library amplification

For short-read sequencing library amplification workflows where polymerase error rate, yield, and coverage uniformity matter.

Amplicon sequencing

For targeted sequencing workflows where amplification accuracy and sequence representation can influence downstream analysis.

Whole-genome sequencing

For short-read workflows where uniform coverage and reduced amplification bias are important across genomic landscapes.

APPLICATIONS

DNA Amplification, PCR/qPCR

DeCodiFi™ Alpha can support high-fidelity amplification of short DNA targets up to 2 kb, particularly when low error rates and accurate template representation are important.

High-Fidelity PCR

For short-amplicon workflows requiring ultra-high fidelity amplification and reduced polymerase-introduced errors.

Genotyping

For research workflows using short amplicons up to 2 kb, where amplification accuracy may affect downstream interpretation.

RESOURCES

Technical Documentation

Technical Data Sheet / Protocol

DeCodi-Fi™ All-In-One Mix TDS / Protocol

VIEW

DeCodi-Fi™ All-In-One Mix TDS / Protocol

VIEW

Brochure

Quick Start Guides

DeCodi-Fi™ All-In-One Mix Quick Start Guide

VIEW

DeCodi-Fi™ PCR Kit Quick Start Guide

VIEW

Testimonials

Kura Biotech's enzyme generates more homogeneous products, requires less template to amplify, and also needs shorter extension times in each amplification cycle. Therefore, it is more efficient and has proofreading activity that enables the generation of more homogeneous products.

Carlos Loncoman, Ph.D.

Assistant Professor, Universidad Austral de Chile

I just tested DeCodi-Fi™ and compared it to the one I regularly use in the lab for cloning. The yield (amount of product) is higher, and without a doubt, I can say that I like the product because it is so simple and fast to work with, while also giving me confidence that it is a high-fidelity enzyme. The kit is very easy to use since everything comes ready to go, which is really convenient.

Cristian Droppelmann, Ph.D.

Research Associate/Team Leader, The University of Western Ontario

I want to highlight the speed of the PCR—it shortens the reaction time by about 20–30 minutes. The PCR product yield is great, making it easy to identify the desired fragment. I'm very satisfied with the product. One of the diseases I study involves a candidate gene with high GC content and pseudogenes, which makes PCR quite challenging. I work with limited amounts of DNA and still achieve high yields of the desired product. When dealing with patient samples, where material is low, that's a major advantage.

Paola Krall, Ph.D.

Academic, Universidad de Chile

Kura Biotech's enzyme is of excellent quality and surpasses many of the options currently available on the market. The objective of the experiment was to generate a plasmid encoding a truncated protein using the overlap PCR technique. The results with DeCodi-Fi™ were very positive: we managed to correctly generate the desired plasmid, and the PCRs were fast and consistent. No errors or aberrant products were observed at any point in the process.

Ignacio Valencia

Researcher, Platech Company

SUPPORT

Frequently Asked Questions

When should I choose DeCodiFi™ Alpha?

Choose DeCodiFi™ Alpha when your workflow requires ultra-high fidelity NGS library amplification, uniform coverage, robust low-input performance, and stability when additional PCR cycles are needed.

How is DeCodiFi™ Alpha different from DeCodiFi™ High-Fidelity Polymerase?

DeCodiFi™ High-Fidelity Polymerase is designed for broader high-fidelity PCR and sequencing workflows. DeCodiFi™ Alpha is designed specifically for ultra-high fidelity NGS library amplification, with emphasis on polymerase error rate, coverage uniformity, low-input performance, and library complexity.

What should I evaluate when choosing a polymerase for NGS library amplification?

Evaluate polymerase error rate, coverage uniformity, GC bias, library yield, input DNA requirements, overcycling tolerance, and compatibility with your downstream sequencing workflow.

Can DeCodiFi™ Alpha help reduce PCR errors in sequencing libraries?

DeCodiFi™ Alpha is designed to reduce polymerase-introduced errors during NGS library amplification. In draft performance data, DeCodiFi™ Alpha was evaluated against multiple high-fidelity polymerase mixes using error per million in Illumina libraries of the complete E. coli genome.

Does DeCodiFi™ Alpha support uniform coverage?

Yes. DeCodiFi™ Alpha is designed to support uniform coverage across genomic landscapes and help reduce amplification bias during library amplification. Draft performance data includes coverage analysis from E. coli Illumina libraries.

Is DeCodiFi™ Alpha suitable for low-input NGS library prep?

DeCodiFi™ Alpha is designed to support low-input library amplification workflows, showing robust library yields from 5 pg DNA under tested conditions.

Is DeCodiFi™ Alpha intended for long-read sequencing?

DeCodiFi™ Alpha is positioned for demanding short-read NGS applications. For long-read sequencing or long-fragment amplification workflows, DeCodiFi™ Long&Complex may be a more relevant starting point.

Can I get technical support for evaluating DeCodiFi™ Alpha?

Yes. Our team can help evaluate whether DeCodiFi™ Alpha fits your NGS library amplification workflow, especially if you are working with low-input DNA, amplification bias, overcycling, or variant-sensitive applications.

DeCodiFi™ Alpha

Accurate Library Prep & High-Fidelity Amplification

Product Overview

Ultra-high fidelity

Uniform coverage

Overcycling stability

Low-input sensitivity

High yield

Fast cycling

Product format

Product format

OPTIMIZED FOR ACCURATE LIBRARY AMPLIFICATION

DeCodiFi™ Alpha

Need a Non-Hot-Start version?

Evidence-based performance

Polymerase error rate in Illumina library amplification

Coverage uniformity across GC content

Sequencing & Library Preparation

DeCodiFi™ Alpha supports sequencing library preparation workflows where ultra-high fidelity, uniform coverage, low-input sensitivity, and library complexity are important for sequencing-ready DNA.

NGS library amplification

Amplicon sequencing

Whole-genome sequencing

DNA Amplification, PCR/qPCR

DeCodiFi™ Alpha can support high-fidelity amplification of short DNA targets up to 2 kb, particularly when low error rates and accurate template representation are important.

High-Fidelity PCR

Genotyping

RESOURCES

Technical Documentation

Technical Data Sheet / Protocol

Brochure

Quick Start Guides

Testimonials

Frequently Asked Questions

When should I choose DeCodiFi™ Alpha?

How is DeCodiFi™ Alpha different from DeCodiFi™ High-Fidelity Polymerase?

What should I evaluate when choosing a polymerase for NGS library amplification?

Can DeCodiFi™ Alpha help reduce PCR errors in sequencing libraries?

Does DeCodiFi™ Alpha support uniform coverage?

Is DeCodiFi™ Alpha suitable for low-input NGS library prep?

Is DeCodiFi™ Alpha intended for long-read sequencing?

Can I get technical support for evaluating DeCodiFi™ Alpha?

Ready to evaluate DeCodiFi™ Alpha?

Related products

DeCodiFi™ High-Fidelity Polymerase

DeCodiFi™ Long&Complex

DeCodiFi™ Plant Direct

DeCodiFi™ Blood Direct