β-glucuronidase from red abalone
β-Glucuronidase solution from Genetically selected Haliotis rufescens (Red Abalone). Stable for 12 months at 2-8°C.
Activity ≥ 100 kU/mL.
For R&D use only. Not for drugs, households, or other uses.
“Greatly improved hydrolysis efficiency enables the reduction of hydrolysis time of incubation from hours to minutes.”
BG100® is a highly efficient and partially purified molluscan β-glucuronidase derived from ocean-fresh Red abalone Haliotis rufescens entrails. This hyperthermophilic β-glucuronidase catalyzes faster hydrolysis reactions. It demonstrates a wider substrate-adaptability and greater efficiency than Helix pomatia, Patella vulgata or even generic abalone-sourced
BG100® is LC-column compatible and enables high-throughput methods. While matrix effects and column-clogging are common issues during LC-MS/MS or HPLC when using impure enzymes or crude preparations, BG100® goes through an advanced purification process which starts with a raw material of ocean-fresh (never freeze-dried) Red abalone entrails. The resulting enzyme preparation, BG100®, is optimized to minimize matrix effects, improve data quality, and achieve short injection-to-injection cycles.
In high-throughput drug-testing, BG100® enables streamlined sample preparation methods including dilute-and-shoot while eliminating the need for protein precipitation and decreasing background noise interference. Improved LOQ and LOD bring further accuracy in therapeutic drug monitoring (TDM), workplace drug testing, forensic drug testing, as well as anti-doping and clinical steroid profiling.
∙ Product form: Liquid
∙ Temperature Range: 55 - 80°C
∙ Optimum temperature: 68°C
∙ pH Range: 4.0 - 5.0
∙ Optimum pH: 4.8
∙ Specific activity: ≥ 100,000 U/mL
∙ Storage/Stability: 2-8°C for 12 months.
∙ Glucuronidase Activity Unit Definition: One unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hour at pH 5.0 and 37°C
∙ Sulfatase Activity Unit Definition: One unit of sulfatase will hydrolyze 1.0 nmol of p-nitrocatechol sulfate per hour at pH 5.0 and 37°C.
Technical DataSheet BG100
BG100 Synthetic Cannabinoids in Urine and Wastewater
BG100 Opiates & Opioids
Publications & Posters
Enzymatic Hydrolysis Efficiency of Glucuronide Conjugates in Human Urine
BG100 Reveals a Hidden Anomaly in Opiate Testing