Product Overview
BG100® is a highly efficient and partially purified molluscan β-glucuronidase derived from ocean-fresh Red abalone Haliotis rufescens entrails. This hyperthermophilic β-glucuronidase catalyzes faster hydrolysis reactions. It demonstrates a wider substrate-adaptability and greater efficiency than Helix pomatia, Patella vulgata or even generic abalone-sourced β-glucuronidase.
AVAILABLE PRESENTATIONS
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- Specifications
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Information
BG100® is superior to other animal β-glucuronidase preparations by its ability to provide comprehensive and complete hydrolysis within 60 minutes. It consistently hydrolyzes all opioid and opiate glucuronides including codeine-6-glucuronide (C6G) with over 87% recovery and has been validated in SAMHSA-certified drug-testing laboratories.Herbal cannabinoids (THC-COOH, THC, CBD, etc.) and benzodiazepines are recovered in the same incubation. BG100® has also been validated with a series of emerging designer drugs such as synthetic cannabinoids (spice, K2, etc.) and synthetic cathinones such as MDPV. In 2013, the US-National Institute on Drug Abuse (NIDA) observed that metabolites of synthetic cannabinoids can be hydrolyzed with 10-fold less β-glucuronidase than with generic abalone preparations, enabling high-throughput synthetic cannabinoid screening.BG100® is LC-column compatible and enables high-throughput methods. While matrix effects and column-clogging are common issues during LC-MS/MS or HPLC when using impure enzymes or crude preparations, BG100® goes through an advanced purification process that starts with a raw material of ocean-fresh (never freeze-dried) Red abalone entrails. The resulting enzyme preparation, BG100®, is optimized to minimize matrix effects, improve data quality, and achieve short injection-to-injection cycles.
In high-throughput drug testing, BG100® enables streamlined sample preparation methods including dilute-and-shoot while eliminating the need for protein precipitation and decreasing background noise interference. Improved LOQ and LOD bring further accuracy in therapeutic drug monitoring (TDM), workplace drug testing, forensic drug testing, as well as anti-doping and clinical steroid profiling.
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Specifications
Product form: Liquid
Temperature Range: 55 - 80°C
Optimum temperature: 68°C
pH Range: 4.0 - 5.0
Optimum pH: 4.8
Specific activity: ≥ 100,000 U/mL
Storage/Stability: 2-8°C for 18 months.
Glucuronidase Activity Unit Definition: One unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hour at pH 5.0 and 37°C
Resources
Technical Data Sheets
Technical Data Sheet BG100
Application Notes
- BG100 TDM
- BG100 Buprenorphine
- BG100 Benzodiazepines
- BG100 THC-COOH
- BG100 Synthetic Cannabinoids in Urine and Wastewater
- BG100 Opiates & Opioids
Publications & Posters
Enzymatic Hydrolysis Efficiency of Glucuronide Conjugates in Human Urine
Case Studies
BG100 Reveals a Hidden Anomaly in Opiate Testing
