Product Overview
There are over 8,000 different β-glucuronidases that have been identified in nature. BGTurbo® can catalyze complete hydrolysis of all glucuronides in just 10 minutes.
Finden’s most loyal battle horse. BGTurbo® is a highly purified recombinant β-Glucuronidase that has demonstrated its capacity to hydrolyze the most difficult and sensitive analytes in 10 minutes at 55°C.
AVAILABLE PRESENTATIONS
- Information
- Specifications
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Information
BGTurbo® High-efficiency Recombinant β-Glucuronidase provides one of the highest hydrolytic activity for the broadest spectrum of conjugated analytes, including “hard to cleave” glucuronides, particularly opiates and opioids such as codeine-6-glucuronide. This ultra-pure aqueous enzyme provides reliable results for high throughput clinical, forensic, workplace drug testing, or other kinds of drug testing laboratories. Flash hydrolysis and sample prep with BGTurbo® eliminate sample preparation bottlenecks while enabling automated hydrolysis and same-day report of accurate and reproducible analytical results.
We have carefully developed BGTurbo® using extensive protein modeling and wet-lab screening to create a massively optimized β-glucuronidase that not only provides one of the fastest hydrolysis but also preserves specimen and peak integrity by eliminating unwanted conversion and minimizing background noise.
Recombinant, high-efficiency BGTurbo® maximizes LC-MS/MS production capacity and return on investment is automation-friendly, and is both exceptionally pure and near-perfectly consistent.
We provide you with the best hydrolysis so you can do the most for the people relying on your answers. That is what BGTurbo® offers you.
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Specifications
Product form: Liquid
Temperature Range: 20-55°C
Optimum temperature: 55°C
pH Range: 6.5-7.2
Optimum pH: 6.8
Specific activity: ≥ 200,000 U/mL
Purity: > 98% β-Glucuronidase
Storage/Stability: 2-8°C for at least 18 months.
Activity Unit Definition: One unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hour at pH 6.8 and 37°C.
CAS No. 9001-45-0
Resources
Technical Data Sheets
Technical Data Sheet BGTurbo
Application Notes
- BGT THC-COOH & minor cannabinoids
- BGT TDM
- BGT TCAs
- BGT Synthetic Cannabinoids in Urine and Wastewater
- BGT Opiates & Opioids
- BGT Opiates & Opioids SAMHSA
- BGT Buprenorphine
- BGT Benzodiazepines
Publications & Posters
- Immobilized Enzymes on Magnetic Beads for Separate Mass Spectrometric Investigation of Human Phase II Metabolites Classes
- Advanced Metabolomics Analysis Through Chemical Biology Tools for the Selective Investigation of Gut Microbiota-Derived Metabolites
- Incomplete hydrolysis of midazolam-glucuronide can cause false negatives on urine drug confirmation by liquid chromatography tandem mass spectrometry
- Complete integration of a fully automated flash hydrolysis protocol of glucuronides in urine with LC-MSMS quantification
- Analysis of Commercially Available Beta-Glucuronidase Enzyme and Optimum Hydrolysis Conditions in Urine
- Hydrolysis efficiency comparison of two beta-glucuronidases: BG100® VS. BGTurbo®
- Streamlining sample preparation with second generation enzymes
- B-One and BGTurbo are user-friendly, facilitating the integration of an enzymatic hydrolysis
- Urine analysis in forensic & clinical toxicology by Martha Concheiro-Guisan, PhD
- Enzymatic and mass spectrometric methodology for the selective investigation of gut microbiota-derived metabolites by Ioanna Tsiara
- Analysis of Benzodiazepines using Liquid Chromatography Mass Spectrometry-Mass Spectrometry by Jonathan T. Tomko
- Prevalence of Cannabidiol, ∆9- and ∆8- Tetrahydrocannabinol and Metabolites in Workplace Drug Testing Urine Specimens
- Exploring Enzymatic Hydrolysis of Urine Samples for Investigation of Drugs Associated with Drug-Facilitated Sexual Assault
Other Resources
- Kura Biotech Benchmark Opiates & Opioids
- BGTurbo Complete Stability Report
- LifeLabs achieves complete Codeine Hydrolysis while avoiding undesirable Analyte Conversions using BGTurbo®
