“Ultra-pure enzyme, thus low protein concentrations, and multiple higher activity for hard-to-cleave analytes across a wide range of drug concentrations.”
There are over 8,000 different β-glucuronidases that have been identified in nature. BGTurbo® can catalyze complete hydrolysis of all glucuronides in just 10 minutes.
Finden’s most loyal battle horse. BGTurbo® is a highly purified recombinant β-Glucuronidase that has demonstrated its capacity to hydrolyze the most difficult and sensitive analytes in 10 minutes at 55°C.
BGTurbo® High efficiency Recombinant β-Glucuronidase, provides one of the highest hydrolytic activity for the broadest spectrum of conjugated analytes, including “hard to cleave” glucuronides, particularly opiates and opioids such as codeine-6-glucuronide. This ultra-pure aqueous enzyme provides reliable results for high throughput clinical, forensic, workplace drug testing or other kinds of drug testing laboratories. Flash hydrolysis and sample prep with BGTurbo® eliminate sample preparation bottle-necks while enabling automated hydrolysis and same-day report of accurate and reproducible analytical results.
We have carefully developed BGTurbo® using extensive protein modeling and wet-lab screening to create a massively optimized β-glucuronidase that not only provides one of the fastest hydrolysis, but also preserves specimen and peak integrity by eliminating unwanted conversion and minimizing background noise.
Recombinant, high-efficiency BGTurbo® maximizes LC-MS/MS production capacity and return-on-investment, is automation friendly, and is both exceptionally pure and near-perfectly consistent.
∙ Product form: Liquid
∙ Temperature Range: 20-55°C
∙ Optimum temperature: 55°C
∙ pH Range: 6.5-7.2
∙ Optimum pH: 6.8
∙ Specific activity: ≥ 200,000 U/mL
∙ Purity: > 98% β-Glucuronidase
∙ Storage/Stability: 2-8°C for at least 18 months.
∙ Activity Unit Definition: One unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hour at pH 6.8 and 37°C.
CAS No. 9001-45-0
Technical DataSheet BGTurbo
BGT THC-COOH & minor cannabinoids
BGT Synthetic Cannabinoids in Urine and Wastewater
BGT Opiates & Opioids
BGT Opiates & Opioids SAMHSA
Publications & Posters
Incomplete hydrolysis of midazolam-glucuronide can cause false negatives on urine drug confirmation by liquid chromatography tandem mass spectrometry
Complete integration of a fully automated flash hydrolysis protocol of glucuronides in urine with LC-MSMS quantification
Analysis of Commercially Available Beta-Glucuronidase Enzyme and Optimum Hydrolysis Conditions in Urine
Hydrolysis efficiency comparison of two beta-glucuronidases: BG100® VS. BGTurbo®
Streamlining sample preparation with second generation enzymes
B-One and BGTurbo are user-friendly, facilitating the integration of an enzymatic hydrolysis
Urine analysis in forensic & clinical toxicology by Martha Concheiro-Guisan, PhD
Enzymatic and mass spectrometric methodology for the selective investigation of gut microbiota-derived metabolites by Ioanna Tsiara
Kura Biotec Benchmark Opiates & Opioids
BGTurbo Complete Stability Report
LifeLabs achieves complete Codeine Hydrolysis while avoiding undesirable Analyte Conversions using BGTurbo®