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B-One®

Room Temp β-glucuronidase for high-throughput analysis

All-in-one β-Glucuronidase stabilized in Buffer solution for Room Temp Hydrolysis.

Store at RT or 2-8°C.

 

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Product Overview

Finden's fastest β-glucuronidase. This 3rd generation recombinant enzyme is specially formulated to provide a faster set-up. B-One® effectively hydrolyzes glucuronides for as fast as an instant to 15 minutes at room temperature, while ensuring an appropriate enzymatic pH in your reaction.

AVAILABLE PRESENTATIONS

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  • Information
  • Specifications
  • Information

    B-One® is designed for high-throughput laboratories that use automated liquid handling instruments. It provides the most efficient hydrolytic activity at room temperature for the broadest spectrum of conjugated analytes.

    This enzyme cleaves codeine-6-glucuronide, dihydrocodeine-6-glucuronide, and other “hard-to-cleave” drug conjugates. It is stabilized in its reaction buffer and hydrolyzes at room temp to save buffer preparation and heating steps; enabling toxicology laboratories to eliminate bottlenecks in sample preparation, and permitting automated hydrolysis and same-day results. 

    B-One® achieves reliable results for high-throughput clinical, forensic, workplace drug testing, and other kinds of laboratories, obtaining complete recoveries as fast as instant to 15 minutes. Samples are ready to analyze in minutes following a simplified Hydrolyze-Analyze™ concept. Because of its high purity, it is free from secondary enzymes and small contaminant molecules to eliminate interferences and unwanted conversions. 

    This unique enzyme does not require supplemental buffering – its “all-in-one” formula allows you to simply add B-One® and ISDs to the urine samples; no additional reagent mixing or cleanup is needed.

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  • Specifications

    Product form: Liquid

    Temperature: Room Temperature (20°C)

    Purity: ≥ 98%

    Storage/Stability: At least 18 months at 2-8°C / or 3 months at RT (20°C)

    Glucuronidase activity: ≥12,000 PS-U/mL

    PS-U Definition: One Product Specific Unit (PS-U) of B-One® will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide in 5 minutes at pH 6.8 and 20°C (Recommended product working conditions).

    CAS No. 9001-45-0

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Resources

Technical Data Sheets

Technical Data Sheet B-One

Application Notes
  • Automated Hydrolysis Extraction and Analysis of Synthetic Cathinones in Urine using a Robotic Autosampler and LC-MS/MS Platform
  • B-One Confirmation of Opiates & Opioids
  • B-One TDM
  • B-One Psilocin
  • B-One THC Hydrolysis in Blood
  • B-One Drug panel testing using LDTD-MS/MS 
  • B-One On-column hydrolysis in Postmortem Urine
  • Benzodiazepines and Metabolites in Urine
Publications & Posters
  • Load Go urine drug testing
  • Evaluation of a new optimized B-glucuronidase for high-throughput laboratories
  • Identification of novel F2-isoprostane metabolites by specific UDP-glucuronosyltransferases
  • Fast analysis of 28 Benzodiazepines and Metabolites
  • B-One and BGTurbo are user-friendly, facilitating the integration of an enzymatic hydrolysis - Boston University.
  • Urine Analysis in Forensic and Clinical Toxicology by Martha Concheiro-Guisan, PhD.
  • Enzymatic hydrolysis of glucuronides in urine at RT for LC-MS-analysis by Joakim Oxelbark
  • Analysis of Substance Use Disorder Patient Samples by QTOF by David Newcombe
  • Assessment of recombinant beta-Glucuronidase vs native beta-Glucuronidase and alkaline conditions for the hydrolysis of THC-glucuronide by Alberto Salomone
  • Best practices in urine drug hydrolysis method
  • Best practices for an enzymatic hydrolysis method of a drug comprehensive panel with opioids
  • Exploring Enzymatic Hydrolysis of Urine Samples for Investigation of Drugs Associated with Drug-Facilitated Sexual Assault
  • Development of an LC–MS/MS method for the determination of five psychoactive drugs in postmortem urine by optimization of enzymatic hydrolysis of glucuronide conjugates
  • Automated enzymatic hydrolysis of urine samples for improved systematic toxicological analysis of drug-facilitated sexual assault cases
  • Optimization of Enzymatic Cleavage of Opiates by β-Glucuronidase in Serum
  • Enzymatic hydrolysis of high codeine levels in urine at RT for LC-MS-analysis
  • Stepping into Room Temperature Hydrolysis for a Comprehensive Panel
  • Metabolism of highly potent synthetic opioid nitazene analogs
  • Human Metabolism of the semi-synthetic cannabinoids HHC, HHCP and their acetates
  • Enzymatic hydrolysis of >10000 (ng/mL) of codeine, morphine, ethylmorphine and oxymorphone glucuronides in urine at RT for LC-MS-analysis by Joakim Oxelbark
  • Enzymatic hydrolysis performance of B-One®: a comprehensive review
  • Flash Room Temp Enzymatic Hydrolysis Protocols for Different Drug Class Using B-One®
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Unsure which enzyme suits your needs? We're here to help!

Unsure which enzyme suits your needs? We're here to help!