B-One®

B-One® is designed for high-throughput laboratories that use automated liquid handling instruments. It provides the most efficient hydrolytic activity at room temperature for the broadest-spectrum of conjugated analytes.

This enzyme cleaves codeine-6-glucuronide, dihydrocodeine-6-glucuronide, and other “hard-to-cleave” drug conjugates. It is stabilized in its reaction buffer and hydrolyzes at room temp to save buffer preparation and heating steps; enabling toxicology laboratories to eliminate bottlenecks in sample preparation, and permitting automated hydrolysis and same-day results. 

B-One® achieves reliable results for high-throughput clinical, forensic, workplace drug testing and other kinds of laboratories, obtaining complete recoveries for as fast as instant to 15 minutes. Samples are ready to analyze in minutes following a simplified Hydrolyze-Analyze™ concept. Because of its high purity, it is free from secondary enzymes and small contaminant molecules to eliminate interferences and unwanted conversions. 

This unique enzyme does not require supplemental buffering – its “all-in-one” formula allows you to simply add B-One® and ISDs to the urine samples; no additional reagent mixing or cleanup is needed. Specifications

∙ Product Form: Liquid

∙ Temperature: Room Temperature (20°C)

∙ Purity: ≥ 98%

∙ Storage/Stability: At least 18 months at 2-8°C / or 3 months at RT (20°C)

∙ Glucuronidase activity: ≥12,000 PS-U/mL

∙ PS-U Definition: One Product Specific Unit (PS-U) of B-One® will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide in 5 minutes at pH 6.8 and 20°C (Recommended product working conditions).

CAS No. 9001-45-0