B-One®

Recombinant
β-Glucuronidase

All-in-one β-Glucuronidase stabilized in Buffer solution for Room Temp Hydrolysis.
Store at 2-8 °C or RT.
For R&D use only. Not for drugs, households, or other uses.

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“Incredibly fast, clean, convenient β-Glucuronidase Hydrolysis at room temperature and automated drug testing compatible.”

B-One®

Finden's fastest ß-glucuronidase. This unique recombinant and highly purified enzyme is clean, stable at room temperature, and doesn’t require heating of samples for hydrolysis. 

B-One® reaches over 90% recoveries within 5 minutes at room temperature.

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Product Overview

Description

B-One® is designed for high-throughput laboratories that use automated liquid handling instruments. This enzyme provides the most efficient hydrolytic activity for the broadest-spectrum of conjugated analytes, giving it a superior ability to cleave codeine-6-glucuronide, dihydrocodeine-6-glucuronide, and other “hard-to-cleave” drug conjugates. Its capacity to hydrolyze in 5 minutes, without a heating step and saving buffer preparation and mixing steps, enables toxicology laboratories to eliminate bottlenecks in sample preparation, permitting automated hydrolysis and same-day results. 

B-One® achieves reliable results for high-throughput clinical, forensic, workplace drug testing and other kinds of laboratories, obtaining complete recoveries within 5 minutes. Samples are ready to analyze in minutes following a simplified Hydrolyze-Analyze™ concept. Because of its high purity, it is free from secondary enzymes and small contaminant molecules, thus eliminating the possibility of interference and unwanted conversions. 

This unique enzyme does not require supplemental buffering – its “all-in-one” formula allows you to simply add B-One® and iSTD to the urine samples; no additional reagent mixing or cleanup is needed. In fact, it’s a reagent.Learn more about the B-One benefitsSpecifications

Product Form: Liquid

Temperature: Room Temperature (20°C)

Purity: ≥ 98%

Storage/Stability: At least 15 months at 2-8°C / or 3 months at RT (20°C)

Glucuronidase activity: ≥12,000 PS-U/mL

PS-U Definition: One Product Specific Unit (PS-U) of B-One™ will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide in 5 minutes at pH 6.8 and 20°C (Recommended product working conditions).

CAS No. 9001-45-0

Available Presentations

     10mL       50mL       100mL   

 

 

Resources

Technical Datasheets

Technical DataSheet B-One

Application Notes

Automated Hydrolysis Extraction and Analysis of Synthetic Cathinones in Urine using a Robotic Autosampler and LC-MS/MS Platform

B-One Codeine

B-One TDM

Publications & Posters

Evaluation of a new optimized β-glucuronidase for flash-hydrolysis

Load Go urine drug testing

Evaluation of a new optimized B-glucuronidase for high-throughput laboratories

 

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