ROOM TEMP β-Gluc for high-throughput analysis

All-in-one β-Glucuronidase stabilized in Buffer solution for Room Temp Hydrolysis.
Store at RT or 2-8 °C.


“Incredibly fast, clean, convenient β-Glucuronidase Hydrolysis at room temperature and automated drug testing compatible.”


Finden's fastest β-glucuronidase.

This 3rd generation recombinant enzyme is specially formulated to provide a faster set up. 

B-One® effectively hydrolyzes glucuronides for as fast as instant to 15 minutes at room temperature, while
ensuring an appropriate enzymatic pH in your





Product Overview


B-One® is designed for high-throughput laboratories that use automated liquid handling instruments. It provides the most efficient hydrolytic activity at room temperature for the broadest-spectrum of conjugated analytes.

This enzyme cleaves codeine-6-glucuronide, dihydrocodeine-6-glucuronide, and other “hard-to-cleave” drug conjugates. It is stabilized in its reaction buffer and hydrolyzes at room temp to save buffer preparation and heating steps; enabling toxicology laboratories to eliminate bottlenecks in sample preparation, and permitting automated hydrolysis and same-day results. 

B-One® achieves reliable results for high-throughput clinical, forensic, workplace drug testing and other kinds of laboratories, obtaining complete recoveries for as fast as instant to 15 minutes. Samples are ready to analyze in minutes following a simplified Hydrolyze-Analyze™ concept. Because of its high purity, it is free from secondary enzymes and small contaminant molecules to eliminate interferences and unwanted conversions. 

This unique enzyme does not require supplemental buffering – its “all-in-one” formula allows you to simply add B-One® and ISDs to the urine samples; no additional reagent mixing or cleanup is needed. Learn more about the B-One benefitsSpecifications

Product Form: Liquid

Temperature: Room Temperature (20°C)

Purity: ≥ 98%

Storage/Stability: At least 18 months at 2-8°C / or 3 months at RT (20°C)

Glucuronidase activity: ≥12,000 PS-U/mL

PS-U Definition: One Product Specific Unit (PS-U) of B-One® will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide in 5 minutes at pH 6.8 and 20°C (Recommended product working conditions).

CAS No. 9001-45-0




Technical Datasheets

Technical DataSheet B-One

Application Notes

Automated Hydrolysis Extraction and Analysis of Synthetic Cathinones in Urine using a Robotic Autosampler and LC-MS/MS Platform

B-One Confirmation of Opiates & Opioids


B-One Psilocin

B-One THC Hydrolysis in Blood

Publications & Posters

Load Go urine drug testing

Evaluation of a new optimized B-glucuronidase for high-throughput laboratories

Identification of novel F2-isoprostane metabolites by specific UDP-glucuronosyltransferases

Fast analysis of 28 Benzodiazepines and Metabolites

B-One and BGTurbo are user-friendly, facilitating the integration of an enzymatic hydrolysis - Boston University.

Urine Analysis in Forensic and Clinical Toxicology by Martha Concheiro-Guisan, PhD.

Enzymatic hydrolysis of glucuronides in urine at RT for LC-MS-analysis by Joakim Oxelbark

Analysis of Substance Use Disorder Patient Samples by QTOF by David Newcombe

Assessment of recombinant beta-Glucuronidase vs native beta-Glucuronidase and alkaline conditions for the hydrolysis of THC-glucuronide by Alberto Salomone

Best practices in urine drug hydrolysis method

Best practices for an enzymatic hydrolysis method of a drug comprehensive panel with opioids

Exploring Enzymatic Hydrolysis of Urine Samples for Investigation of Drugs Associated with Drug-Facilitated Sexual Assault

Development of an LC–MS/MS method for the determination of five psychoactive drugs in postmortem urine by optimization of enzymatic hydrolysis of glucuronide conjugates

Automated enzymatic hydrolysis of urine samples for improved systematic toxicological analysis of drug-facilitated sexual assault cases

Optimization of Enzymatic Cleavage of Opiates by β-Glucuronidase in Serum

KURA_B-One-100mL 2

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