Product Overview
Kura Biotech's fastest β-glucuronidase. This 3rd generation recombinant enzyme is specially formulated to provide a faster set-up. B-One® effectively hydrolyzes glucuronides for as fast as an instant to 15 minutes at room temperature, while ensuring an appropriate enzymatic pH in your reaction.
AVAILABLE PRESENTATIONS
10mL 50mL 100mL 250mL
- Information
- Specifications
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Information
B-One® is designed for high-throughput laboratories that use automated liquid handling instruments. It provides the most efficient hydrolytic activity at room temperature for the broadest spectrum of conjugated analytes.
This enzyme cleaves codeine-6-glucuronide, dihydrocodeine-6-glucuronide, and other “hard-to-cleave” drug conjugates. It is stabilized in its reaction buffer and hydrolyzes at room temp to save buffer preparation and heating steps; enabling toxicology laboratories to eliminate bottlenecks in sample preparation, and permitting automated hydrolysis and same-day results.
B-One® achieves reliable results for high-throughput clinical, forensic, workplace drug testing, and other kinds of laboratories, obtaining complete recoveries as fast as instant to 15 minutes. Samples are ready to analyze in minutes following a simplified Hydrolyze-Analyze™ concept. Because of its high purity, it is free from secondary enzymes and small contaminant molecules to eliminate interferences and unwanted conversions.
This unique enzyme does not require supplemental buffering – its “all-in-one” formula allows you to simply add B-One® and ISDs to the urine samples; no additional reagent mixing or cleanup is needed.
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Specifications
Product form: Liquid
Temperature: Room Temperature (20°C)
Purity: ≥ 98%
Storage/Stability: At least 18 months at 2-8°C / or 3 months at RT (20°C)
Glucuronidase activity: ≥12,000 PS-U/mL
PS-U Definition: One Product Specific Unit (PS-U) of B-One® will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide in 5 minutes at pH 6.8 and 20°C (Recommended product working conditions).
CAS No. 9001-45-0
Resources
Technical Data Sheets
Technical Data Sheet B-One
Application Notes
- Automated Hydrolysis Extraction and Analysis of Synthetic Cathinones in Urine using a Robotic Autosampler and LC-MS/MS Platform
- B-One Confirmation of Opiates & Opioids
- B-One TDM
- B-One Psilocin
- B-One THC Hydrolysis in Blood
- B-One Drug panel testing using LDTD-MS/MS
- B-One On-column hydrolysis in Postmortem Urine
- Room Temperature Hydrolysis with B-One and One-Step Cleanup with Beta-Gone
Publications & Posters
- Load Go urine drug testing
- Evaluation of a new optimized B-glucuronidase for high-throughput laboratories
- Identification of novel F2-isoprostane metabolites by specific UDP-glucuronosyltransferases
- Fast analysis of 28 Benzodiazepines and Metabolites
- B-One and BGTurbo are user-friendly, facilitating the integration of an enzymatic hydrolysis - Boston University.
- Urine Analysis in Forensic and Clinical Toxicology by Martha Concheiro-Guisan, PhD.
- Enzymatic hydrolysis of glucuronides in urine at RT for LC-MS-analysis by Joakim Oxelbark
- Analysis of Substance Use Disorder Patient Samples by QTOF by David Newcombe
- Assessment of recombinant beta-Glucuronidase vs native beta-Glucuronidase and alkaline conditions for the hydrolysis of THC-glucuronide by Alberto Salomone
- Best practices in urine drug hydrolysis method
- Best practices for an enzymatic hydrolysis method of a drug comprehensive panel with opioids
- Exploring Enzymatic Hydrolysis of Urine Samples for Investigation of Drugs Associated with Drug-Facilitated Sexual Assault
- Development of an LC–MS/MS method for the determination of five psychoactive drugs in postmortem urine by optimization of enzymatic hydrolysis of glucuronide conjugates
- Automated enzymatic hydrolysis of urine samples for improved systematic toxicological analysis of drug-facilitated sexual assault cases
- Optimization of Enzymatic Cleavage of Opiates by β-Glucuronidase in Serum
- Enzymatic hydrolysis of high codeine levels in urine at RT for LC-MS-analysis
- Stepping into Room Temperature Hydrolysis for a Comprehensive Panel
- Metabolism of highly potent synthetic opioid nitazene analogs
- Human Metabolism of the semi-synthetic cannabinoids HHC, HHCP and their acetates
- Enzymatic hydrolysis of >10000 (ng/mL) of codeine, morphine, ethylmorphine and oxymorphone glucuronides in urine at RT for LC-MS-analysis by Joakim Oxelbark
- Enzymatic hydrolysis performance of B-One®: a comprehensive review
- Flash Room Temp Enzymatic Hydrolysis Protocols for Different Drug Class Using B-One®
- Characterizing the Sulfated and Glucuronidated (Poly)phenol
Metabolome for Dietary Biomarker Discovery - Direct analysis in real time mass spectrometry (DART-MS/MS) for rapid urine opioid detection in a clinical setting
- Rapid quantitative screening of 15 synthetic cannabinoids in urine using direct analysis in real time tandem mass spectrometry: Screening method validation and cross-correlation study with liquid chromatography tandem mass spectrometry