“Incredibly fast, clean, convenient β-Glucuronidase Hydrolysis at room temperature and automated drug testing compatible.”
Finden's fastest ß-glucuronidase. This unique recombinant and highly puriﬁed enzyme is clean, stable at room temperature, and doesn’t require heating of samples for hydrolysis.
B-One® reaches over 90% recoveries within 5 minutes at room temperature.
B-One® is designed for high-throughput laboratories that use automated liquid handling instruments. This enzyme provides the most efficient hydrolytic activity for the broadest-spectrum of conjugated analytes, giving it a superior ability to cleave codeine-6-glucuronide, dihydrocodeine-6-glucuronide, and other “hard-to-cleave” drug conjugates. Its capacity to hydrolyze in 5 minutes, without a heating step and saving buffer preparation and mixing steps, enables toxicology laboratories to eliminate bottlenecks in sample preparation, permitting automated hydrolysis and same-day results.
B-One® achieves reliable results for high-throughput clinical, forensic, workplace drug testing and other kinds of laboratories, obtaining complete recoveries within 5 minutes. Samples are ready to analyze in minutes following a simplified Hydrolyze-Analyze™ concept. Because of its high purity, it is free from secondary enzymes and small contaminant molecules, thus eliminating the possibility of interference and unwanted conversions.
This unique enzyme does not require supplemental buffering – its “all-in-one” formula allows you to simply add B-One® and iSTD to the urine samples; no additional reagent mixing or cleanup is needed. In fact, it’s a reagent.Specifications
∙ Product Form: Liquid
∙ Temperature: Room Temperature (20°C)
∙ Purity: ≥ 98%
∙ Storage/Stability: At least 12 months at 2-8°C / or 3 months at RT (20°C)
∙ Glucuronidase activity: ≥12,000 PS-U/mL
∙ PS-U Definition: One Product Specific Unit (PS-U) of B-One® will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide in 5 minutes at pH 6.8 and 20°C (Recommended product working conditions).
CAS No. 9001-45-0
Technical DataSheet B-One
Automated Hydrolysis Extraction and Analysis of Synthetic Cathinones in Urine using a Robotic Autosampler and LC-MS/MS Platform
Publications & Posters
Load Go urine drug testing
Evaluation of a new optimized B-glucuronidase for high-throughput laboratories
Fast analysis of 28 Benzodiazepines and Metabolites
B-One and BGTurbo are user-friendly, facilitating the integration of an enzymatic hydrolysis - Boston University.
Urine Analysis in Forensic and Clinical Toxicology by Martha Concheiro-Guisan, PhD.
Enzymatic hydrolysis of glucuronides in urine at RT for LC-MS-analysis by Joakim Oxelbark
Analysis of Substance Use Disorder Patient Samples by QTOF by David Newcombe
Assessment of recombinant beta-Glucuronidase vs native beta-Glucuronidase and alkaline conditions for the hydrolysis of THC-glucuronide by Alberto Salomone