A hot-start high-fidelity DNA polymerase designed to amplify long DNA fragments up to 44 kb, with high yield, fast cycling, and low bias from low-input samples.
Up to 44 kb amplification
64% lower error vs. KAPA™ HiFi
Fast cycling: under 15 sec/kb
Low-input compatibility
25%–85% GC compatibility
Uracil tolerance
Formats: 2X All-In-One Mix
High-Fidelity PCR Kit
Amplifies up to 44 kb, delivering high-yield templates ready for long-read sequencing platforms.
Amplifies complex DNA from as little as 0.1 ng and medium-complexity templates from 5 pg.
More than 60% reduction in error rates, delivering high-accuracy amplification for precision-critical applications.
Uniform amplification and high DNA recovery, even from challenging or GC-rich templates (25%–85% GC).
Achieves rapid amplification with extension speeds under 15 sec/kb.
Efficiently reads through uracil, supporting both methylation analysis and protocols involving damaged DNA.
PERFORMANCE DATA
Maximize target yield with highly efficient amplification, even with fragment sizes up to 44 kb.
Amplification of lambda DNA fragments 20 kb and 44 kb with DeCodiFi™ High-Fidelity polymerase, KAPA HiFi, Platinum SuperFi II, and Q5. Each target was amplified from low input (1ng). Reactions were performed following the optimized conditions for each enzyme and using 24 cycles. The experiment was run in triplicate.
DeCodiFi™ supports amplification of long DNA targets from limited template input, including 17.6 kb beta globin amplification tested across decreasing DNA input amounts.
Amplification of the 17.6 kb human beta globin gene was performed using DeCodiFi™ High-Fidelity Polymerase and KOD Xtreme™ Hot Start DNA Polymerase. Each target was amplified from different inputs in a 25 μL reaction: 1000 pg, 125 pg, and 50 pg. A no-template control (0 pg) was also included. All reactions were performed following the optimized conditions for each enzyme and using 30 cycles. The experiment was run in triplicate.
DeCodiFi™ showed a 64% reduction in error rates compared to KAPA™ HiFi in an Illumina library-based fidelity comparison.
Comparison of DeCodiFi™, KAPA HiFi, and Toyobo HiFi, measured in terms of fold improvement over Taq Polymerase, based on Illumina libraries of the complete E. coli genome.
DNA concentration obtained from 25 μL of lambda 15 kb PCR reaction using DeCodiFi™ PCR kit, DeCodiFi™ All-in-One Mix, and competitors' enzymes (Kapa HiFi, Platinum SuperFi II, and Q5). The experiment was run in triplicate.
DeCodiFi™ supports low-bias amplification across tested common, AT-rich, and GC-rich genomes, helping preserve more uniform sequence coverage in sequencing library workflows.
Illumina libraries prepared from a common genome (Escherichia coli) were amplified using Taq polymerase, KAPA HiFi PCR kit, and DeCodiFi™ High-Fidelity PCR kit (left). Illumina libraries prepared from an AT-rich genome (Staphylococcus epidermidis) and a GC-rich genome (Streptomyces leeuwenhoekii) were amplified using Taq polymerase, KAPA HiFi PCR kit, and DeCodiFi™ High-Fidelity PCR kit (right).
APPLICATIONS
For workflows where longer DNA fragments and low-input amplification are important.
For targeted sequencing workflows that require accurate and consistent amplification.
For workflows where amplification quality and sequence representation can influence downstream genome analysis.
APPLICATIONS
For routine and demanding amplification workflows where accuracy and yield matter.
For long, complex, or high GC templates where high-fidelity amplification, yield, and cycling efficiency are important.
