
From field to PCR. Skip the purification.
DeCodi-Fi™ Direct Plant is an All-in-One Mix formulated to enable high-fidelity PCR directly from crude plant extracts, no purification needed. It tolerates inhibitors like polyphenols and polysaccharides, delivering consistent results across diverse species and tissues. Powered by the DeCodi-Fi™ high-fidelity polymerase, it delivers the precision, yield, and consistency that define the DeCodi-Fi™ kit family. Ideal for genotyping, transgene detection, and high-throughput plant workflows.

From field to PCR. Skip the purification.
DeCodi-Fi™ Direct Plant is an All-in-One Mix formulated to enable high-fidelity PCR directly from crude plant extracts, no purification needed. It tolerates inhibitors like polyphenols and polysaccharides, delivering consistent results across diverse species and tissues. Powered by the DeCodi-Fi™ high-fidelity polymerase, it delivers the precision, yield, and consistency that define the DeCodi-Fi™ kit family. Ideal for genotyping, transgene detection, and high-throughput plant workflows.
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High inhibitor tolerance: Handles plant inhibitors like polyphenols and polysaccharides, enabling reliable amplification directly from crude extracts, no purification required.
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Diverse plant types and tissues: Validated across a variety of crops and inhibitor-heavy species, including corn, soybean, wheat, and strawberry.
- Multiplex ready: Supports simultaneous amplification of multiple targets in a single reaction, ideal for high-throughput genotyping and screening.
- Genotyping by sequencing
- Multiplex PCR
-
High inhibitor tolerance: Handles plant inhibitors like polyphenols and polysaccharides, enabling reliable amplification directly from crude extracts, no purification required.
-
Diverse plant types and tissues: Validated across a variety of crops and inhibitor-heavy species, including corn, soybean, wheat, and strawberry.
- Multiplex ready: Supports simultaneous amplification of multiple targets in a single reaction, ideal for high-throughput genotyping and screening.
- Genotyping by sequencing
- Multiplex PCR
KEY PERFORMANCE DATA
AMPLIFY DIVERSE PLANT TYPES AND TISSUES +
Strong and consistent amplification across diverse plant samples, including challenging species such as strawberry.
Comparison of direct PCR amplification (template size: 326 bp) across various plant types using DeCodi-FiT™ and competitor polymerases. HotSHOT crude extracts were prepared from leaf (L) and seed (S) tissues of wheat, corn, soybean, tomato, strawberry, and potato. Reactions (25 μL) contained 20% HotSHOT extract and were run at Tm 58°C for 35 cycles. 1 μL of PCR product was loaded per lane, with (+) 5 ng purified DNA as the positive control and NTC as the no-template control.
MULTIPLEX CAPABILITY +
Enables multiplex PCR directly from crude plant samples, allowing simultaneous target detection.
Multiplex PCR amplification of ten target fragments (124, 172, 252, 365, 503, 648, 761, 920, 1094, and 1261 bp) from wheat DNA using DeCodi-Fi™ Plant Direct and a positive control (10 ng of purified wheat DNA). All reactions were performed in triplicate, with 0.1 μL of PCR product loaded per lane.
SHORTENED WORKFLOW +
Skip purification, save time on sample prep, and reduce consumables and reagent use.
PRODUCT FORMAT

DeCodi-Fi™ Plant Direct
2X All-In-One Mix![]()
Provides our DeCodi-Fi™ high-fidelity polymerase in a 2X All-in-One MasterMix optimized for crude plant extracts. This ready-to-use format includes all essential PCR components: dNTPs, MgCl₂, enhancers, and stabilizers, within a proprietary buffer designed to tolerate common plant inhibitors like polyphenols and polysaccharides. Just add primers and sample.
Product Type: All-in-One/MasterMix
Polymerase: DeCodi-Fi™ Hotstart High-Fidelity Polymerase
Format: 5 mL
Reactions: 400
Storage: Store at -20°C
RELATED PRODUCTS
Ready to amplify your research?
Take your DNA sequencing to the next level with DeCodi-Fi™. Contact our team for sales and technical support.
Ready to amplify your research?
Take your DNA sequencing to the next level with DeCodi-Fi.
Contact our team for sales and technical support.




