DeCodi-Fi™ High Fidelity Polymerase Kits

La enzima de Blikka genera productos más homogéneos, requiere menos ADN molde para amplificar y necesita tiempos de extensión más cortos en cada ciclo de amplificación. Por lo tanto, es más eficiente y posee actividad de corrección de errores (proofreading), lo que permite obtener productos aún más uniformes.

Carlos Loncoman, PhD

Facultad de Ciencias Veterinarias | Universidad Austral de Chile

I just tested DeCodi-Fi and compared it to the one I regularly use in the lab for cloning (Phusion High-Fidelity DNA Polymerase from Thermo). The yield (amount of product) is higher than with Phusion, and without a doubt, I can say that I like the product because it is so simple and fast to work with, while also giving me confidence that it is a high-fidelity enzyme. Your kit is very easy to use since everything comes ready to go, which is really convenient.

Cristian Droppelmann, PhD

Molecular Medicine Group | The University of Western Ontario

Quiero destacar la velocidad del producto: reduce el tiempo de reacción en aproximadamente 20 a 30 minutos. El rendimiento es excelente, lo que facilita la identificación del fragmento deseado. Estoy muy feliz con el producto.

Una de las enfermedades que estudio involucra un gen candidato con alto contenido de GC y pseudogenes, lo que hace que el PCR sea bastante desafiante. Trabajo con cantidades limitadas de ADN y aun así obtengo altos rendimientos del producto deseado. Al trabajar con muestras de pacientes, donde el material es escaso, eso representa una gran ventaja.

Paola Krall, PhD.

Facultad de Medicina | Universidad de Chile

Blikka Genomics enzyme is of excellent quality and surpasses many of the options currently available on the market. The objective of the experiment was to generate a plasmid encoding a truncated protein using the overlap PCR technique. The results with DeCodi-Fi were very positive: we managed to correctly generate the desired plasmid, and the PCRs were fast and consistent. No errors or aberrant products were observed at any point in the process. This experiment was performed in parallel with a Pfu enzyme. While both enzymes worked correctly, DeCodi-Fi showed a clear advantage: it did not generate non-specific fragments, unlike the Pfu, which did produce unwanted products of different sizes.

Ignacio Valencia

Researcher | Platech Company