• How long will Instant Buffer I last once I dissolve it with dH20?
Instant buffer I is formulated to last for one year upon dilution in LCMS Grade water. However, we recommend to check the buffer for contamination/floating debris on a daily basis.
• Can I store prepared Instant Buffer I at 2-8 °C?
We recommend storing prepared Instant Buffer I at room temperature, because it can precipitate out of solution when stored in cold temperatures. If refrigerated, make sure crystals are properly solubilized before using it by agitating gently at room temperature until precipitate dissolves.
• If the enzyme I have in my lab has expired, what are the risks of using it anyway?
The risk is that the enzyme will not have its optimum activity. You can measure activity of the enzyme or contact Kura Biotech at firstname.lastname@example.org to see if a retest of the lot in question is possible.
• What should I do if my enzyme has been subjected to improper storage conditions?
Please contact Kura Biotech. We can help you determine if the enzyme can be used or needs to be replaced.
• What are the shelf life and storage conditions of Kura's enzymes?
∙ B-One: 12 months at 2-8°C or 3 months at Room Temperature
• Can I increase the volume of sample?
Sure! Just be sure to keep a reasonable enzyme:sample ratio for your analyte. For more information please talk with a hydrolysis expert at email@example.com.
• What happens if I use a pH and Temperature outside of the range suggested?
The enzyme will be working in sub-optimum condition negatively impacting the ability to cleave substrates and you will obtain less recovery. If you need a specific parameter, please contact a hydrolysis expert at firstname.lastname@example.org to help you set up an optimal protocol.
• Does the protocol suggested in the Technical Datasheet of your enzymes apply for any drug class?
No, it doesn't. The protocol of our Technical Datasheets seeks to hydrolyze the hardest-to-cleave analytes, not being necessary to use same enzyme volume, or temperature, or time of incubation for easier to cleave analytes. Please try to find the drug class you will be analyzing in the Applications Page to find the Application Note for each case.
• May I hydrolyze in the sample preparation columns/plates? (SPE/SLE/Filter)
Assuming the sample prep device has an appropriate hydrophobic frit to hold up the reaction, at temperature, for the entire incubation, yes. The use of a purified enzyme avoids protein precipitation and centrifugation steps, normally necessary when working in presence of high content of protein. Be careful, many SPE or SLE devices will start to flow with gravity at room temperature, while the temperature increases, the flow increases too.
• How can I ensure I am getting the best performance from my enzyme?
Contant one of our hydrolysis experts at email@example.com or call (800) 332-1448.
• Sometimes, a B-glucuronidase cannot hydrolyze glucuronides conjugates. Why?
Hydrolysis can be impacted by many variables in protocol or matrix. It is important to optimize protocols for time, temperature, and pH. Considerations need to be made for reaction components such as MeOH, endogenous inhibitors, isomers having steric hindrances as well as non specific binding to surfaces. Kura Hydrolysis experts are ready to help you with specific metabolite and matrix questions. Please reach out to firstname.lastname@example.org or call (800) 332-1448.
• What recovery rates can I expect from incubating hardest to cleave analytes such as opiates/opioids using BGTurbo or B-One?
According to previous experiences, it is possible to obtain over 90% recovery of opiates/opioids such as codeine-6-glucuronide up to 2,500 ng/mL, using:
∙ BGTurbo 2:5 enzyme - urine ratio within 10 min at 55°C.
∙ B-One 1:1 enzyme - urine ratio within 10 min at room temperature.
• What if I want increased B-One enzyme but not increased dilution of the sample?
The current B-One formulation is proposed for common laboratory practices, if your laboratory has a specific condition that requires more enzyme without further dilution, contact a us at email@example.com or call (800) 332-1448.
• How can I protect the labile analytes during my hydrolysis?
Labile analytes like morphine can be protected by using prurified enzymes, short incubation times, and mild temperatures.
• How can I place an order?
If you're ordering in USA or Canada:
1. You can place an order on our webshop.
2. Fill out this form to contact sales.
3. Mail your PO to firstname.lastname@example.org.
If you're order is international:
1. Fill out this form.
2. Mail your PO to email@example.com.
• How is the purchase order process of Kura Biotech?
Email your PO to firstname.lastname@example.org. If you require credit terms, we may require a credit check.
• What happens if I don't receive a PO confirmation?
You can send an email to email@example.com and request a formal order acknowledgment.
• How can I track my order?
We usually ship with FedEx. Once you receive your tracking number, you can track your shipment at https://www.fedex.com/apps/fedextrack/. If you haven’t received the tracking number within the standard lead time, please feel free to email firstname.lastname@example.org and ask us.
• Can I get free samples?
Yes, we provide free samples so you can perform validation. Please use the following form to request a sample:
• What should I do if I receive a different order?
Please let us know immediately at email@example.com or by phone at +1 (800) 332 1448 or +1 (760) 955 9010
• What should I do if I receive an invoice with incorrect information/prices/quiantities/etc?
Should there be any issues with your invoice, please contact us at your earliest convenience at firstname.lastname@example.org or by phone at +1 (800) 332 1448 or +1 (760) 955 9010.
• Can I place an order if I have unpaid invoices?
No, we will reject any order from a customer with a past-due balance. If you need to pay overdue invoices, please contact email@example.com or contact +1 (800) 332 1448 or +1 (760) 955 9010.
• What payment methods do you accept?
Bank transfer, check, credit card. Please note that checks should be mailed to our USA office.
• Are Kura Biotech's payment conditions negotiable?
Yes, they are. If you need more information please contact firstname.lastname@example.org.
• How long is the lead time for my order to arrive?
The standard lead time for retail orders within the US is 2-5 business days.
For orders outside of the US please contact email@example.com for lead times.
• What happens if the shipment arrives with broken or damaged products?
You can ask for a replacement of the product (no shipping charges) or a credit note for the broken or damaged product.
• Which shipping companies does Kura Biotech use?
We usually use FedEx, but we occasionally use UPS or DHL as well. If you prefer a specific shipping method, please let us know.
• How much are the shipping costs?
∙ Standard Shipping: $60 USD
Region: UK (exclusive), Ireland (exclusive), Scandinavia (Norway, Denmark, Sweden) exclusive, Nordic Countries (Finland, Iceland), France, Germany, Finland, Turkey, Switzerland, Czech Republic, Spain.
Region: Germany, Luxembourg, Belgium, Netherlands, Austria, Switzerlad.