RESIST® Food

Salmonella
enterica

RESIST Food allows the detection of the presence or absence of Salmonella enterica from surface samples and a variety of food quickly, with high sensitivity and specificity. RESIST Food is based on vanguard molecular biology whose principle is isothermal amplification and fluorescence, complemented by visual interpretation without highly complex equipment.

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TECHNICAL DESCRIPTION

RESIST Food Salmonella enterica Detection Kit is a device for the detection of the Salmonella enterica genome in a fast and straightforward way. The technique is based on rapid extraction of the DNA in samples and subsequent isothermal amplification of the target genetic material. One of the main characteristics of this kit is that the results can be obtained without the need for highly complex equipment since the visualization of the presence is coupled to a fluorimetric reaction, appreciable in a blue light transilluminator with 470 nm wavelengths.

COMPONENTS

Components	Description
2X RESIST Food Premix	Enzymatic reagent used to perform isothermal amplification.
Primer Mix L. monocytogenes	Reagent that includes the primers and visualizer coupled to the amplification.
Positive control DNA	Synthetic genetic material used as a positive control.
Nuclease-free water	Nuclease-free water used as a negative control.
Blink Fast Extraction	Reagent used for the extraction of genetic material.

PRODUCT SPECIFICATION:

Product format: 100 reactions

Storage temperature: -20 °C

Detection limit: 1 CFU/μL

Analytical detection limit: 10 copies/reaction

Type of result: Qualitative

Results time: 45 minutes after completion of the enrichment methodology.

Enrichment time: 18 - 24 hrs

Compatible equipment: Blue light transilluminator, real-time equipment with HEX channel and P51 XD

Compatible Enrichment Media: Buffered peptone water

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