ENGINEERED TdT VARIANT

TdT for natural nucleotides

Engineered TdT for natural nucleotide incorporation

TdT for natural nucleotides is an engineered terminal deoxynucleotidyl transferase designed to efficiently incorporate natural or modified deoxynucleotides (ddNTPs) to the 3' end of DNA strands.

Built for researchers developing high-efficiency extension, homopolymer tailing, 3’ end labeling, this TdT gives teams a high-performance option for synthesis using natural and common modified nucleotides (e.g., ddNTP).

Natural nucleotide and modified nucleotides (ddNTP) incorporation

DNA end extension workflows

DNA homopolymer tailing

DNA end-labeling (ddNTP)

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Why TdT A

Built for natural nucleotide workflows

TdT for natural nucleotides is best positioned for applications where natural nucleotide compatibility is the main requirement.

Engineered for reliable DNA modification

Specifically designed for consistent performance in homopolymer tailing, 3’ end labeling with modified nucleotides, and supporting established methods like TUNEL assays and TdT-dependent PCR.

Technical support included

Discuss your nucleotide, substrate, reaction conditions, and application goals with our team.

Product format

OPTIMIZED FOR ACCURATE LIBRARY AMPLIFICATION

DeCodi-Fi™ Alpha
2X All-In-One Mix

Provides our DeCodi-Fi™ high-fidelity polymerase in a 2X MasterMix optimized for NGS library amplification. This mix includes all essential PCR components: dNTPs, MgCl₂, and stabilizers, within a proprietary buffer designed to enhance fidelity and coverage. Just add primers and template.

Product Type: All-In-One / MasterMix
Polymerase: DeCodiFi™ Alpha Hotstart High-Fidelity Polymerase
Format: 5 mL
Reactions: 400
Storage: -20°C

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Choose your starting point

If your workflow is centered on natural nucleotides, TdT A is the focused standalone enzyme to evaluate. If you are still comparing nucleotide chemistries or not sure which engineered TdT variant fits best, start with the TdT Synthesis Panel.

TdT A

Standalone engineered TdT variant

Best for:
Teams working with natural or modified nucleotide (ddNTPs) incorporation.

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TdT Synthesis Panel

Six engineered TdT variants for workflow screening

Best for:
Teams that are still comparing enzyme behavior across nucleotide types, substrate structures, or application conditions

Evaluate the panel

Not sure yet? Start with the panel.

If you are comparing nucleotide types, working across multiple substrates, or unsure whether TdT for natural nucleotides is the right starting point, begin with the TdT Synthesis Panel to evaluate multiple engineered variants before choosing a standalone enzyme.

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PERFORMANCE DATA

Evidence-based performance

Polymerase error rate in Illumina library amplification

DeCodiFi™ Alpha shows an ultra-high fidelity profile in an Illumina library-based comparison of polymerase error rates, supporting workflows where polymerase-introduced errors can affect downstream interpretation.

HIGHER FIDELITY

Comparison of Kura DeCodiFi™ Alpha 2X All-In-One Mix, Kura DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, Watchmaker Equinox Library Amplification Kit, Roche KAPA HiFi HotStart ReadyMix, and Taq, measured in error per million based on Illumina libraries of the complete E. coli genome.

Comparison of DeCodiFi™ Alpha 2X All-In-One Mix, Kura DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, Watchmaker Equinox Library Amplification Kit, Roche KAPA HiFi HotStart ReadyMix, and Taq, measured in error per million based on Illumina libraries of the complete E. coli genome.

Coverage uniformity across GC content

DeCodiFi™ Alpha supports uniform coverage across tested genomic regions, helping reduce GC-related coverage bias during NGS library amplification.

UNIFORM COVERAGE
Illumina libraries prepared from an E. coli genome were amplified using Kura DeCodiFi™ Alpha 2X All-In-One Mix, Kura DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, and Taq polymerase.

Illumina libraries prepared from an E. coli genome were amplified using DeCodiFi™ Alpha 2X All-In-One Mix, DeCodiFi™ 2X All-In-One Mix, Q5® High-Fidelity 2X Master Mix, and Taq polymerase.

APPLICATIONS

Natural nucleotide incorporation & DNA extension workflows

TdT for natural nucleotides supports workflows where natural or modified nucleotide incorporation, extension behavior, and reaction optimization are central to method development.For precise DNA labeling using modified nucleotides such as ddNTPs to create high-quality probes and markers.

Homopolymer tailing

For the efficient addition of poly-nucleotide tails (e.g., Poly-A) to the 3' ends of DNA, essential for cloning and sequencing preparation.

3’ End labeling

For precise DNA labeling using modified nucleotides such as ddNTPs to create high-quality probes and markers.

RESOURCES

Technical Documentation

Technical Data Sheet / Protocol

DeCodi-Fi™ All-In-One Mix TDS / Protocol

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DeCodi-Fi™ All-In-One Mix TDS / Protocol

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Brochure

Quick Start Guides

DeCodi-Fi™ All-In-One Mix Quick Start Guide

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DeCodi-Fi™ PCR Kit Quick Start Guide

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Testimonials

Kura Biotech's enzyme generates more homogeneous products, requires less template to amplify, and also needs shorter extension times in each amplification cycle. Therefore, it is more efficient and has proofreading activity that enables the generation of more homogeneous products.

Carlos Loncoman, Ph.D.

Assistant Professor, Universidad Austral de Chile

I just tested DeCodi-Fi™ and compared it to the one I regularly use in the lab for cloning. The yield (amount of product) is higher, and without a doubt, I can say that I like the product because it is so simple and fast to work with, while also giving me confidence that it is a high-fidelity enzyme. The kit is very easy to use since everything comes ready to go, which is really convenient.

Cristian Droppelmann, Ph.D.

Research Associate/Team Leader, The University of Western Ontario

I want to highlight the speed of the PCR—it shortens the reaction time by about 20–30 minutes. The PCR product yield is great, making it easy to identify the desired fragment. I'm very satisfied with the product. One of the diseases I study involves a candidate gene with high GC content and pseudogenes, which makes PCR quite challenging. I work with limited amounts of DNA and still achieve high yields of the desired product. When dealing with patient samples, where material is low, that's a major advantage.

Paola Krall, Ph.D.

Academic, Universidad de Chile

Kura Biotech's enzyme is of excellent quality and surpasses many of the options currently available on the market. The objective of the experiment was to generate a plasmid encoding a truncated protein using the overlap PCR technique. The results with DeCodi-Fi™ were very positive: we managed to correctly generate the desired plasmid, and the PCRs were fast and consistent. No errors or aberrant products were observed at any point in the process.

Ignacio Valencia

Researcher, Platech Company

SUPPORT

Frequently Asked Questions

What is TdT for natural nucleotides?

TdT for natural nucleotides is a standalone engineered terminal deoxynucleotidyl transferase designed for workflows centered on natural or modified nucleotide (dNTPs) incorporation. It is intended for researchers who want a focused enzyme format for DNA extension, method development, and assay optimization using standard nucleotide substrates.

What is TdT for natural nucleotides best suited for?

TdT for natural nucleotides is best suited for applications involving natural dNTPs and NTPs, especially when the goal is to optimize extension performance, refine reaction conditions, or continue development after identifying a preferred enzyme from our TdT synthesis panel.

Does TdT for natural nucleotides work with natural nucleotides?

.Yes. TdT for natural nucleotides is positioned for workflows based on natural nucleotides, making it a strong option for researchers who are not primarily working with blocked nucleotide chemistries.

Does TdT for natural nucleotides work with modified nucleotides?

Yes. TdT for natural nucleotides is positioned for workflows based on common modified nucleotides such as ddNTPs. If you are working with other chemistries, we recommend starting with the TdT Synthesis Panel or discussing your application with our team.

Is TdT for natural nucleotides intended for blocked nucleotides?

TdT for natural nucleotides should not be positioned as the lead choice for blocked nucleotide workflows. If your application depends on 3′-amino blocked nucleotides or repeated deblocking cycles, TdT for blocked is the more appropriate product to highlight. If you are working with other chemistries, we recommend starting with the TdT Synthesis Panel or discussing your application with our team.

When should I choose TdT for natural nucleotides instead of the full TdT panel?

TdT for natural nucleotides is a good choice when you have already identified it as the right fit for your workflow and want to continue development with a single enzyme. If you are still comparing variants or exploring which TdT best matches your chemistry, the TdT Synthesis Panel is usually the better starting point.

What kinds of workflows can TdT for natural nucleotides support?

TdT for natural nucleotides can support:

Homopolymer tailing: Adding poly-N tails (A, T, C, G) to the 3’ ends of DNA.
DNA end-labeling: Creating probes and markers using modified nucleotides like ddNTPs or DIG-dUTP.
Apoptosis detection: Serving as the core enzyme for sensitive TUNEL assays.
Template-independent amplification: Facilitating TdT-dependent PCR and RACE protocols.
Standard synthesis development: Building methods that require efficient incorporation of natural dNTPs and NTPs.

Is TdT for natural nucleotides a good fit for method development?

Yes. TdT for natural nucleotides is well-suited for method development when your workflow depends on natural nucleotide use, and you want to reduce complexity by working with a single engineered TdT.

Can TdT for natural nucleotides be used after panel screening?

Yes. TdT for natural nucleotides is a practical next step for teams that started with the TdT panel and want to move into a standalone format for continued optimization, repeat experiments, or routine use.

How is TdT for natural nucleotides different from TdT for blocked nucleotides?

TdT for natural nucleotides is best positioned for natural and modified nucleotide workflows, while TdT for blocked nucleotides is better suited for 3′-amino blocked nucleotide incorporation and iterative synthesis workflows. If your work depends on reversible blocking strategies, TdT for blocked nucleotides is likely the stronger fit.

Can I request help selecting the right TdT?

Yes. If you share your workflow, nucleotide type, and development goals, our team can help you determine whether TdT for natural nucleotides, TdT for blocked nucleotides , or the TdT Synthesis Panel is the best fit.